Authentication of paprika using HPLC-UV fingerprints

Publication date: Available online 13 February 2020Source: LWTAuthor(s): Xavier Cetó, Cristina Sánchez, Núria Serrano, José Manuel Díaz Cruz, Oscar NúñezAbstractIn this work we combine simple extraction and HPLC-UV methodologies with chemometric pattern-recognition strategies in order to obtain characteristic fingerprints of phenolic compounds that allow the authentication of paprika samples. To illustrate the potential of the proposed approach, two different adulteration scenarios were considered, namely adulteration of paprika based on its type (sweet, bittersweet and spicy) as well as on its region (Murcia, la Vera and Czech Republic). Upon preparation of a proper set of samples, they were analysed using a C18 reversed-phase column and registered chromatograms were then compressed employing fast Fourier transform (FFT) to reduce the large dimensionality of the data set, while preserving all relevant features. Next, data were analysed using linear discriminant analysis (LDA) for the qualitative discrimination of adulterated samples, followed by partial least-squares regression (PLS) modelling to quantitatively assess the adulteration degree.Graphical abstract
Source: LWT Food Science and Technology - Category: Food Science Source Type: research