Knockdown of hsa_circ_0058124 inhibits the proliferation of human lung cancer cells by up-regulation of miR-1297.

Knockdown of hsa_circ_0058124 inhibits the proliferation of human lung cancer cells by up-regulation of miR-1297. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):584-593 Authors: Li X, Zhang Q, Yang Z Abstract Hsa_circ_0058124 was reported to possess the capacity of enhancing tumorigenesis and invasiveness. This investigation explored the effects of hsa_circ_0058124 on human lung cancer. qRT-PCR was employed for assessing the expression of hsa_circ_0058124 and miR-1297. Cell transfection was conducted for altering the expression of hsa_circ_0058124 and miR-1297. Dual-luciferase reporter gene assay was employed for exploring the relationship between hsa_circ_0058124 and miR-1297. CCK-8 assay, colony formation, flow cytometry, western blot and transwell assay were respectively conducted for exploring the effects of hsa_circ_0058124 silencing and miR-1297 inhibition. The expression of proteins participated in PTEN/AKT and Wnt/β-catenin pathways were determined for exploring the underlying mechanism. Hsa_circ_0058124 was highly expressed in human lung tumour tissues. Besides, hsa_circ_0058124 silencing suppressed cell viability, colony formation, migration and invasion, while enhanced cell apoptosis, which were respectively verified by the regulation of apoptosis-associated and metastasis-related proteins. Additionally, hsa_circ_0058124 silencing inhibited the expression of proteins involved in PTEN/AKT and Wnt/β-catenin pathways includ...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research