Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman ’s Syndrome

Asherman’s syndrome (AS) is characterized by intrauterine adhesion or fibrosis resulting from damage to the endometrium, often leading to amenorrhea, infertility, or recurrent pregnancy loss. Although various therapeutic strategies for AS have been proposed, the options remain limited. New strategies such as bone marrow-derived mesenchymal stem cell (BM-MSC) therapy aim to potentiate the intrinsic capacity of endometrial regeneration. However, BM-MSC therapy has not been widely adopted mainly because it involves invasive and expensive procedures such as bone marrow biopsy and cell storing. On the other hand, platelet-rich plasma (PRP) is considered safe and affordable because it involves the less invasive procedure of blood collection from peripheral veins to produce PRP. To assess the effectiveness of human PRP infusion for endometrial regeneration, we established a murine model of injury-induced AS and evaluated endometrial morphology, expression of fibrosis-related factors, implantation sites (IS), and pregnancy outcomes associated with human PRP treatment. We found that treatment with human PRP was associated with improved endometrial morphology, reduced degree of fibrosis, and down-regulated expression of fibrosis-related factors in murine model of AS. Furthermore, human PRP treatment was associated with a higher number of IS and live-births. Our results suggest that human PRP treatment may become a valuable strategy to promote the regeneration of damaged endometri...
Source: Frontiers in Physiology - Category: Physiology Source Type: research

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ez A Abstract OBJECTIVES: Relationships between weight and fertility are well known. The aim of this study is to assess the prevalence of lifetime eating disorder (ED) in a sample of infertile women seeking a specific infertility treatment, pulsatile gonadotropin-releasing hormone (pGnRH) treatment, and to compare it to the prevalence of lifetime ED in a sample of infertile women seeking other types of assisted reproductive technology (ART) treatments. DESIGN: Non-randomized, observational study including infertile female patients. Two-group design including consecutive women treated with GnRH pump (pGnRH) or...
Source: Eating and weight disorders : EWD - Category: Eating Disorders & Weight Management Authors: Tags: Eat Weight Disord Source Type: research
The reproductive function of humans is regulated by several sex hormones which are secreted in synergy with the circadian timing of the body. Sleep patterns produce generic signatures that physiologically drive the synthesis, secretion, and metabolism of hormones necessary for reproduction. Sleep deprivation among men and women is increasingly reported as one of the causes of infertility. In animal models, sleep disturbances impair the secretion of sexual hormones thereby leading to a decrease in testosterone level, reduced sperm motility and apoptosis of the Leydig cells in male rats. Sleep deprivation generates stressful...
Source: Journal of Circadian Rhythms - Category: Biology Source Type: research
Editor’s Note: For more on the challenges as well as strategies to address physician infertility, please read this Academic Medicine Invited Commentary, cowritten by the author of this blog post. Most of us in medicine pride ourselves on being organized, detail-oriented, conscientious and able to achieve excellent results with enough hard work—and are not used to failure and loss of control. My experience with infertility has been the first time that I truly experienced failure and complete loss of control, realizing that fertility is not a merit-based system. No matter how hard I try, how many do...
Source: Academic Medicine Blog - Category: Universities & Medical Training Authors: Tags: Featured Guest Perspective infertility physician well-being women in academic medicine Source Type: blogs
AbstractPurpose of ReviewYoung women represent a minority of breast cancer patients for which fertility, family planning, and pregnancy represent unique vulnerabilities. This review intends to discuss recent published evidence regarding treatment-related infertility, fertility counseling, and preservation.Recent FindingsFertility concerns are common among young women with breast cancer and may negatively affect treatment decisions. Data is available to aid providers in approximating odds of post-treatment amenorrhea and infertility. Multiple fertility preservation techniques are available. While embryo preservation is most...
Source: Current Breast Cancer Reports - Category: Cancer & Oncology Source Type: research
ConclusionLow-dose contraceptive treatment containing progesterone, followed by Drospil, which includes both estradiol and progesterone, had a synergistic effect that led to the growth of the patient ’s uterus.
Source: Journal of Medical Case Reports - Category: General Medicine Source Type: research
Rational: The growing population of young cancer survivors and a trend toward postponing pregnancy until later years in life are leading to a deeper attention towards understanding treatment-induced sequelae, and, in particular, the effects of cancer and/or treatment on fertility. Nowadays, the infertility risks potentially associated with molecular targeted therapies are not established, and clinical reports are sparse. Moreover, the increasing use of molecular targeted drugs in the adjuvant setting and in diseases with better prognosis makes preservation of fertility a major topic in current research. Patient's concer...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Clinical Case Report Source Type: research
Conclusions: These 6 cases demonstrated the positive effect of a combined approach, using acupuncture and herbs to reduce DOR and regulate hormonal disorders, creating sustainable environments for conceiving and maintaining pregnancy, thus, translating into clinical success. Clinical observation of these cases sheds new light on designing future clinical trials and practice of TCM for practitioners who provide infertility treatments. PMID: 31871529 [PubMed]
Source: Acupuncture in Medicine - Category: Complementary Medicine Authors: Tags: Med Acupunct Source Type: research
ConclusionsHysteroscopy may play an important role before or in conjunction with assisted reproductive techniques to help infertile women and couples achieve their goals of pregnancy and live birth of a child.
Source: Journal of Medical Case Reports - Category: General Medicine Source Type: research
AbstractObjectiveTo evaluate the hormonal profile, antral follicle count (AFC) and ovarian response of patients with hypogonadotropic hypogonadism (HH).DesignObservational retrospective cohort including infertile women with HH undergoing assisted reproductive treatment (ART).SettingUniversity-affiliated infertility center.Patient(s)Thirty-three women with HH who underwent ART between January 2007 and September 2018. The control group comprised 66 age-matched counterparts with tubal or male factor infertility. The patients with an abnormal karyotype, and those presenting primary or secondary amenorrhea due to other causes, ...
Source: Journal of Assisted Reproduction and Genetics - Category: Reproduction Medicine Source Type: research
Primary ovarian insufficiency (POI) refers to ovarian loss of function under the age of 40 years and lead those patients to usually present by amenorrhea and infertility. One of the reasons of POI is chemotherapy for cancer patients. It is broadly believed that chemotherapy drugs may vastly eliminate granulosa cells which are essential for oocyte survival and follicular development. Our previous study shows that transplantation of human bone marrow derived mesenchymal stem cell (hBM-MSC) in chemotherapy induced POI mouse ovary can reverse POI through correction of serum hormonal level, promote follicular generation in ovar...
Source: Fertility and Sterility - Category: Reproduction Medicine Authors: Tags: Poster session Source Type: research
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