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Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news

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Publication date: Available online 14 February 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Hong Chen, Qianru Zhang, Ruimin Chen, Xin Yuan, Xiangquan Lin, Xiaohong Yang, Ying ZhangAbstractCongenital adrenal hyperplasia (CAH) encompasses a group of autosomal recessive diseases characterized by enzymatic defects in steroid synthesis. Lipoid congenital adrenal hyperplasia (LCAH) is the most severe form of CAH, insofar as the initial step of steroid synthesis is impaired. Variants in the steroid acute regulatory (STAR) gene are responsible for LCAH. To describe the clinical and genetic charac...
Source: The Journal of Steroid Biochemistry and Molecular Biology - Category: Biochemistry Source Type: research
Publication date: Available online 14 February 2020Source: Protein Expression and PurificationAuthor(s): Meng Xu, Matthew J. Bailey, Jennifer Look, Francois BaneyxAbstractThe Car9 affinity tag is a dodecameric silica-binding peptide that can be fused to the N- and C-termini of proteins of interest to enable their rapid and inexpensive purification on underivatized silica in a process that typically relies on l-lysine as an eluent. Here, we show that silica paper spin columns and borosilicate multi-well plates used for plasmid DNA purification are suitable for recovering Car9-tagged proteins with high purity in a workflow c...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
This study has suggested that the β-CD-GO could play an efficient and beneficial source of the adsorbent for the purpose of eliminating Cd(II) from aqueous solution.Graphical abstract
Source: Process Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 15 February 2020Source: Plant Physiology and BiochemistryAuthor(s): Seyed Morteza Zahedi, Marjan Sadat Hosseini, Javier Abadía, Mina MarjaniAbstractThe increasing salinity in soils and irrigation water is a major concern for growers of strawberry, a salt-sensitive horticultural crop. The hormone melatonin (N-acetyl-5-methoxytryptamine) is involved in many biological processes and affects plant responses to environmental stresses. The effects of weekly 100 and 200 μM melatonin sprays on leaf composition parameters (photosynthetic pigment and macronutrient concentrations, oxidat...
Source: Plant Physiology and Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 15 February 2020Source: Plant Physiology and BiochemistryAuthor(s): Dong Huang, Mengnan Ma, Qian Wang, Maoxue Zhang, Guangquan Jing, Chao Li, Fengwang MaAbstractArbuscular mycorrhizal fungi (AMF) can form a symbiotic relationships with most terrestrial plants and play an important role in plant growth and adaptation to various stresses. To study the role of AMF in regulating drought resistance in apple, the effects of drought stress on Malus hupehensis inoculated with AMF were investigated. Inoculation of AMF enhanced apple plants growth. Mycorrhizal plants had higher total chlorophyll co...
Source: Plant Physiology and Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 14 February 2020Source: Plant Physiology and BiochemistryAuthor(s): Ming-Shuang Zhong, Han Jiang, Yue Cao, Yong-Xu Wang, Chun-Xiang You, Yuan-Yuan Li, Yu-Jin HaoAbstractDrought can activate many stress responses in plant growth and development, including the synthesis of epidermal wax and the induction of abscisic acid (ABA), and increased wax accumulation will improve plant drought resistance. Therefore, an examination of wax biosynthesis genes could help to better understand the molecular mechanism of environmental factors regulating wax biosynthesis and the wax associated stress respon...
Source: Plant Physiology and Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 14 February 2020Source: MitochondrionAuthor(s): Jennifer A. McElhoe, Mitchell M. HollandAbstractImproved resolution of massively parallel sequencing (MPS) allows for the characterization of mitochondrial (mt) DNA heteroplasmy to levels previously unattainable with traditional sequencing approaches. An essential criterion for the reporting of heteroplasmy is the ability of the MPS method to distinguish minor sequence variants (MSVs) from system noise, or error. Therefore, an assessment of the background noise in the MPS method is desirable to identify the point at which reliable data can b...
Source: Mitochondrion - Category: Biochemistry Source Type: research
This study was designed to investigate changes in blood subfatin and spexin levels during gestational diabetes mellitus (GDM) and childbirth, and define the mechanisms of these hormones in the physiopathology of GDM. A total of 60 pregnant women, comprising 30 diagnosed with GDM and 30 with normal gestation, were included in the study. The diagnosis of GDM was made through a 75-g oral glucose tolerance test (OGTT) administered between 24 and 28 weeks of pregnancy. The amounts of subfatin, spexin, and insulin were measured in blood samples by enzyme-linked immunosorbent assays; lipid profiles, glucose, and other biochemical...
Source: Peptides - Category: Biochemistry Source Type: research
Publication date: May 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 189Author(s): Xiangyu Zhang, Mingcong Wu, Yu Zhang, Jie Zhang, Jin Su, Chunrong YangAbstractMolecularly-targeted nanobubbles (NBs) offer opportunities to improve the ability of ultrasound imaging to identify specific pathological tissue from healthy tissue. In this work, we aimed to design ligands-conjugated, nanosized, lipid ultrasound contrast agents (UCAs) and apply the agents in the ultrasound imaging of atherosclerotic plaque. Anti-VEGFR-2 ligands were conjugated to UCAs using the noncovalent biotin-avidin linker method. Several investiga...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
ConclusionFor the first time our study demonstrates the distinct action of E. carinatus and N. naja venoms on the process of NETosis. NETosis being a newly explored area in snake venom pharmacodynamics, it is important to study its impact on the various pathophysiological properties induced by snake venoms.SignificanceUnderstanding the varied actions of snake venoms on neutrophils/blood cells and the role of DNase are likely to provide insights for better management of snakebite pathophysiology.
Source: Biochimica et Biophysica Acta (BBA) General Subjects - Category: Biochemistry Source Type: research
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