Expression of attack and growth phase genes of Bdellovibrio bacteriovorus in the presence of Gram-negative and Gram-positive prey

Publication date: Available online 13 February 2020Source: Microbiological ResearchAuthor(s): M. Waso, S. Khan, W. Ahmed, W. KhanAbstractThe expression of attack phase (AP) and growth phase (GP) genes of Bdellovibrio bacteriovorus (B. bacteriovorus) was compared in the presence of Gram-negative [Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae)] and Gram-positive [Enterococcus faecium (E. faecium)] prey, using relative quantitative polymerase chain reaction (relative qPCR) assays. The genes bd0108 (pili retraction/extrusion) and merRNA (massively expressed riboswitch RNA) were highly expressed in the AP cells [3.99- to 6.06-fold (E. coli), 3.91- to 7.05-fold (K. pneumoniae) and 2.91- to 7.30-fold (E. faecium)]. The fliC1 gene (flagella filament) was also expressed at a high level in the AP cells however, after 240 min of co-culture with E. faecium the expression of fliC1 remained low (at 0.759-fold), while in the presence of the Gram-negative prey fliC1 expression increased. Additionally, the GP genes bd0816 (peptidoglycan-modifying enzyme) and groES1 (chaperone protein) were not induced in the presence of E. faecium. However, they were expressed in the early GP and GP of B. bacteriovorus after exposure to the Gram-negative prey. It can thus be concluded that B. bacteriovorus senses the presence of potential prey when exposed to Gram-positive and Gram-negative bacteria, however the GP genes are not induced in co-culture with E. faecium. The results f...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research

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The intestinal tract is the largest digestive organ in the human body. It is colonized by, and consistently exposed to, a myriad of microorganisms, including bifidobacteria, lactobacillus, Escherichia coli, enterococcus, clostridium perfringens, and pseudomonas. To protect the body from potential pathogens, the intestinal tract has evolved regional immune characteristics. These characteristics are defined by its unique structure, function, and microenvironment, which differ drastically from those of the common central and peripheral immune organs. The intestinal microenvironment created by the intestinal flora and its prod...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Conclusion: This study has a total complication rate of 16%, approximately 10% of those are severe. The most common complication to PCNL was infection (60%), followed by bleeding (5.4%), reoperation (1.6%) and pain (0.5%). The high prevalence of E. faecalis might need to be considered, however the results should be validated in a larger cohort, possibly with a higher rate of antibiotic resistance, before a change of guidelines regarding prophylactic antibiotics could be proposed. PMID: 32208808 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Urology - Category: Urology & Nephrology Tags: Scand J Urol Source Type: research
CONCLUSION: The results indicated that E. faecium isolates from spontaneously fermented sausage showed a potential for further investigation and possible application as probiotics. PMID: 32222865 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - Category: Biotechnology Authors: Tags: Biotechnol Lett Source Type: research
Antibiotic treatment of patients undergoing complex medical treatments can deplete commensal bacterial strains from the intestinal microbiota, thereby reducing colonization resistance against a wide range of antibiotic-resistant pathogens. Loss of colonization resistance can lead to marked expansion of vancomycin-resistant Enterococcus faecium (VRE), Klebsiella pneumoniae, and Escherichia coli in the intestinal lumen, predisposing patients to bloodstream invasion and sepsis. The impact of intestinal domination by these antibiotic-resistant pathogens on mucosal immune defenses and epithelial and mucin-mediated barrier integ...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
s T Abstract Resistance to the "last resort" antibiotics, such as carbapenems, has led to very few antibiotics being left to treat infections by multi-drug resistant bacteria. Spread of carbapenem resistance (CR) has been well characterised for the clinical environment. However, there is lack of information about its environmental distribution. Our study first reveals that CR is present in a wide range of Gram-negative bacteria in the coastal seawater environment, including four phyla, eight classes, and 30 genera. These bacteria were likely introduced into seawater via stormwater flows. Some CR isolates...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Abstract The aim of this study was to optimize the mechanical and texture properties of edible film improving its antibacterial property after adding rosemary essential oil (REO) using a Doehlert matrix. Films with the highest mechanical properties were acquired using a polymer composition of 65.2% glycerol, 24.3% gelatin, 10.0% chitosan and 0.5% pectin. This composition provided the highest elongation at break, tensile strength and texture values, which were respectively 51.60 ± 6.04%, 8.53 ± 2.36 MPa and 13.67 ± 1.43. The antibacterial activity of REO enriched films against Bac...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
Conclusion: The observed widespread multidrug resistance clearly warrant implementing stricter control measures, local guidelines of antimicrobials usage, and continuous epidemiological surveys at hospitals and communities. PMID: 32148678 [PubMed]
Source: Iranian Journal of Microbiology - Category: Microbiology Tags: Iran J Microbiol Source Type: research
Authors: Panphut W, Budsabun T, Sangsuriya P Abstract Long pepper (Piper retrofractum Vahl) is a Thai medicinal herb which has been used as one of the common ingredients in variety of Thai foods. Here, we investigated antimicrobial activities of crude bioactive metabolites extracted from fruits of P. retrofractum against 10 pathogenic organisms (bacteria and yeast) causing opportunistic infections in human or animals including Bacillus subtilis ATCC6633, Staphylococcus aureus ATCC25923, Enterococcus faecalis ATCC2921, Escherichia coli ATCC25922, Klebsiella pneumonia TISTR1843, Pseudomonas aeruginosa ATCC741, Salmon...
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
Authors: Devi SP, Singh RKH, Sujata W, Joshi DD Abstract The synthesis and characterization on four rhodium(II) complexes with the formula [Rh2(CH3COO)2(AMUH)2(dcda)2](CH3COO)2(1),[Rh2(CH3COO)2(AEUH)2(dcda)2](CH3COO)2(2),[Rh2(CH3COO)2(APrnUH)2(dcda)2](CH3COO)2(3),[Rh2(CH3COO)2(ABnUH)2(dcda)2](CH3COO)2(4), where AMUH = 1-amidino-O-methylurea, AEUH = 1-amidino-O-ethylurea, APrnUH = 1-amidino-O-n-propylurea, ABnUH = 1-amidino-O-n-butylurea, dcda = dicyandiamide are reported. The complexes were prepared by the reaction of dicyandiamide with rhodium(I...
Source: Nucleosides, Nucleotides and Nucleic Acids - Category: Biochemistry Tags: Nucleosides Nucleotides Nucleic Acids Source Type: research
Conclusions: Bacterial infection prevalence is relatively high in patients with liver cirrhosis. Although all analyzed scores, including the LMR, NLR, aspartate aminotransferase (AST)/alanine aminotransferase (ALT), CRP, CTP, and MELD, allowed the prediction of bacterial occurrence, the LMR had the highest clinical utility, according to the area under the curve (AUC) and odds ratio (OR).
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
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