Absolute quantitation of high abundant Fc-glycopeptides from human serum IgG-1.

In this study, eleven high abundant IgG-1 Fc-glycopeptides with definite peptide sequences and glycoforms were purified from commercial IgG protein by using two-dimensional hydrophilic interaction liquid chromatographic system. Based on the acquired glycopeptide standards, an absolute quantitation strategy was developed to determine the concentrations of 11 target IgG-1 glycopeptides from pooled human sera. A wide range of Fc-glycopeptide concentrations from 0.60 to 17.61 nmol mL-1 was achieved with excellent accuracy and reproducibility from pooled human sera IgG-1. Compared to conventional relative quantitation, this strategy provides more accurate distribution profiles of 11 high abundant Fc-glycopeptides and degree of glycosylation from pooled human sera IgG-1.
PMID: 32043992 [PubMed - in process]

Source: Analytica Chimica Acta - February 13, 2020 Category: Chemistry Authors: Cao C, Yu L, Fu D, Yuan J, Liang X Tags: Anal Chim Acta Source Type: research