Ratiometric fluorescent sensing system for drug residue analysis: Highly sensitive immunosensor using dual-emission quantum dots hybrid and compact smartphone based-device.

Ratiometric fluorescent sensing system for drug residue analysis: Highly sensitive immunosensor using dual-emission quantum dots hybrid and compact smartphone based-device. Anal Chim Acta. 2020 Mar 15;1102:91-98 Authors: Yu W, Jiang C, Xie B, Wang S, Yu X, Wen K, Lin J, Wang J, Wang Z, Shen J Abstract Immunoassays such as the enzyme-linked immunosorbent assay (ELISA) are utilized extensively for detecting protein biomarkers and small molecules in healthcare, environmental monitoring, and food analysis. Unfortunately, the current strategies for immunoassays often require sophisticated apparatus such as a microplate reader, which might not be available in resource-limited areas. To mitigate this problem, we designed a compact smartphone based-device and a multicolor response immunosensor. First, we designed a compact and cost-effective 3D-printed attachment, where a light-emitting diode was used as a light excitation source and a smartphone captured the fluorescent emission signals. Second, by combining quantum dots hybrid and chemical redox reaction, multiple color responses were displayed in the presence of the analyte at different concentrations. Third, solutions with distinct tonality could be readily distinguished by the naked eye and they were suitable for quantitative analysis using the hue-saturation-lightness color space based on a smartphone application. The versatility of the proposed sensing system was demonstrated by imple...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research