CD160 expression on CD8 + T cells is associated with active effector responses but limited activation potential in pancreatic cancer

In this study, we investigated the effects of CD160 on CD8+ T cells in pancreatic cancer. First, we found that the frequency of PD-1+ cells was comparable between CD160+ and CD160−CD8+ T cells, with the former presenting significantly higher PD-1 expression level. In contrast, the frequency of TIM-3+ cells was higher among CD160+ cells but the expression level was comparable between CD160+ and CD160−CD8+ T cells. The IFN- γ and IL-2-expressing CD8+ T cells, directly ex vivo, were highly enriched in the CD160+ subset. However, when CD160+ and CD160−CD8+ T cells were stimulated, the proliferation levels of CD160+ and CD160− cells were initially comparable, but were significantly lower in CD160+CD8+ T cells than in CD160−CD8+ T cells later on. The IFN- γ and IL-2 transcription levels were initially higher in CD160+CD8+ T cells, but eventually reduced in CD160+CD8+ T cells compared to CD160−CD8+ T cells. Also, CD160+CD8+ T cells presented lower cytotoxic capacity than CD160−CD8+ T cells. Interestingly, we observed that tumor-infiltrating CD8+ T cells were significantly enriched with the CD160+ subset in pancreatic cancer patients. In addition, patients with higher frequencies of tumor CD160+CD8+ T cells presented lower survival. Overall, these data demonstrated that tumor-infiltrating CD8+ T cells were enriched with the CD160+ subset in pancreatic cancer, with active effector responses directly ex vivo but limited potential for further activation.
Source: Cancer Immunology, Immunotherapy - Category: Cancer & Oncology Source Type: research