Prevalence and antimicrobial resistance of Campylobacter isolated from carcasses of chickens slaughtered in Poland – a retrospective study

Publication date: Available online 12 February 2020Source: Food ControlAuthor(s): Kinga Wieczorek, Łukasz Bocian, Jacek OsekAbstractThe aim of the present study was to assess the prevalence of Campylobacter on chicken carcasses tested in Poland during 2014–2018 and to evaluate the antimicrobial susceptibility of the recovered isolates. The results were compared to the previous similar investigations performed in 2009–2013 [Wieczorek K. &Osek J. (2015). A five-year study on prevalence and antimicrobial resistance of Campylobacter from poultry carcasses in Poland. Food Microbiology, 49, 161–165]. A total of 2,367 swab samples from broiler carcasses were collected by official veterinarians in abattoirs where chickens were slaughtered for commercial purposes located in all 16 voivodeships (administrative provinces) of Poland. Susceptibility tests against ciprofloxacin, erythromycin, gentamycin, nalidixic acid, streptomycin, and tetracycline were performed using the minimal inhibitory concentration method. Campylobacter spp. was identified by culture and confirmed by PCR methods in 1,263 (53.4%) chicken carcasses. The prevalence of the bacteria ranged from a minimum of 49.8% in 2014 to a maximum of 58.6% in 2015. The analogous previous study showed that in the period of 2009–2013, 54.4% out of 2,114 chicken carcass samples were positive for Campylobacter. All Campylobacter isolates identified in the present investigation were classified to C. coli (738...
Source: Food Control - Category: Food Science Source Type: research

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This study aimed to determine the prevalence, antibiotic resistance patterns and source tracking of clinical C. jejuni isolates from paediatric diarrhoeal patients in Pakistan.MethodsA total of 150 stool samples from children were processed for the presence of C. jejuni using culture, biochemical tests and species-specific PCR. Antibiotic susceptibility of the isolates was determined by the disc diffusion method, and metallo-β-lactamase (MBL) producers were detected using gene-specific PCR. Source tracking was performed using source-predictive PCR.ResultsC. jejuni was present in 54.6% of the processed samples. More th...
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Publication date: Available online 10 October 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Bárbara Martín-Maldonado, Laura Montoro-Dasi, Maria Teresa Pérez-Gracia, Jaume Jordá, Santiago Vega, Francisco Marco-Jiménez, Clara MarinAbstractWild birds have repeatedly been found to be involved in the dissemination of enteric bacterial pathogens in the environment. The aim of this study was to determine the occurrence of Salmonella and Campylobacter as well as the antimicrobial resistance in wild Bonelli’s eagles nestlings in Eastern Spain. In addition...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
ménez F, Marin C Abstract Wild birds have repeatedly been found to be involved in the dissemination of enteric bacterial pathogens in the environment. The aim of this study was to determine the occurrence of Salmonella and Campylobacter as well as the antimicrobial resistance in wild Bonelli's eagles nestlings in Eastern Spain. In addition, we compared the efficiency of two sampling methods (fresh faecal samples from nest and cloacal swabs from nestlings) for detection of both bacteria. A total of 28 nests with 45 nestlings were analysed. In the nest, Salmonella occurrence was 61 ± 9.2%...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Abstract Campylobacter jejuni and C. coli are leading causes of human foodborne illness with poultry as a major vehicle. Turkeys are frequently colonized with Campylobacter but little is known about Campylobacter survival in turkey feces, even though fecal droppings are major vehicles for Campylobacter within-flock transmission as well as for environmental dissemination. Our objective was to examine survival of Campylobacter, including different strains, in freshly-excreted feces from naturally-colonized commercial turkey flocks and in suspensions of turkey feces in water from the turkey house. Fecal and water sus...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Publication date: Available online 23 July 2019Source: Journal of Global Antimicrobial ResistanceAuthor(s): Zobia Noreen, Fariha Siddiqui, Sundus Javed, Brendan W. Wren, Habib BokhariAbstractObjectivesCampylobacter jejuni has been classified as a member of priority pathogens group due to the rapid emergence of multidrug resistant isolates. In the present study we planned to determine the prevalence, antibiotic resistance patterns and source tracking of clinical C. jejuni isolates from pediatric diarrheal patients in Pakistan.MethodsA total of 150 stool samples from children were processed for the presence of Campylobacter ...
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
The objective of this study was to assess the molecular diversity, using flaA sequencing, of 602 C. jejuni isolated from chicken food chain, i.e. chicken feces (n = 151), chicken carcasses (n = 150), chicken meat (n = 150) and from humans (n = 151) and to determine antimicrobial multiresistant profiles of the isolates as well as to analyze the relationship of the isolate genotypes with their antimicrobial resistance profiles and source of isolation. Multidrug resistant patterns were identified in 110 (18.3%) C. jejuni isolates recovered from all sources and most isolates were resistant to ciprofloxacin (CIP), nalidixic aci...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 29 April 2019Source: Food ControlAuthor(s): Xinfeng Han, Xingai Guan, Hang Zeng, Jiakang Li, Xuelin Huang, Yiping Wen, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Sanjie Cao, Rui Wu, Xiaoping Ma, Likou ZouAbstractA total of 220 Campylobacter isolates was recovered from 656 samples along a commercial duck slaughter line after a panel of confirmation tests including selective culture methods, biochemical identification and multiplex PCR in southwestern China. The prevalence of Campylobacter spp. was 33.5%, including 16.6% of Campylobacter jejuni, 15.7% of Campylobacter coli and 3.6% of o...
Source: Food Control - Category: Food Science Source Type: research
Conclusion Genomic analyses herein performed allowed us to confirm the recently (Pérez-Cataluña et al., 2018a,b) suggested amendment of A. butzleri as Al. butzlerii, comb. nov. Antimicrobial susceptibility tests defined Ab 55 and Ab 6V strains as resistant to vancomycin, tetracyclin, nalidixic acid (only Ab 55 whereas Ab 6V is intermediate resistant), erythromycin, and β-lactam antibiotics. Moreover, in our strains isolated from shellfish, we identified numerous virulence, antibiotic, and heavy metal resistance determinants, also additional to those previously found in the genome sequenced A. butzleri ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, association rule mining is an effective tool for identifying patterns of multidrug resistance within antimicrobial susceptibility testing data and evaluating the statistical and biological significance of the patterns. Rule quality measures used to sort and differentiate rules should be tested using resampling procedures to minimize the false discovery rate. Rule mining identified consistent multidrug resistance patterns involving beta-lactams, sulfisoxazole, tetracycline, gentamicin, and streptomycin in E. coli isolated from chicken carcasses and meat between 2004 and 2012. The generally low rule overlap su...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion The present results demonstrated that Chilean salmon farms play an important role as reservoirs of sulfonamide- and trimethoprim-resistant bacteria. The prevalence of conjugative plasmids and integrons among sul-carrying bacteria suggests these bacteria, mainly belonging to the Pseudomonas genus may contribute to high spread of bacterial resistance to sulfonamides and other antibacterials in environments associated with Chilean salmon farms. This is the first study reporting the occurrence of transferable sul and dfr genes and integrons among the antimicrobial resistant bacteria associated with Chilean salmonid...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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