Nanostructured transition metal chalcogenide embedded on reduced graphene oxide based highly efficient biosensor for cardiovascular disease detection

Publication date: June 2020Source: Microchemical Journal, Volume 155Author(s): Dipti Chauhan, Pooja, Vishakha Nirbhaya, Chandra Mohan Srivastava, Ramesh Chandra, Suveen KumarAbstractHerein, we report nanostructured metal chalcogenide (molybdenum tetraselenide, nMo3Se4) embedded on reduced graphene oxide (rGO) based electrochemical immunosensor for cardiovascular disease biomarker [cardiac troponin I (cTnI)] detection. The nanostructured molybdenum tetraselenide-reduced graphene oxide (nMo3Se4-rGO) was synthesized via low temperature hydrothermal method and further functionalization was carried out using 3-aminopropyltriethoxy silane (APTES). Deposition of functionalized nanostructured material (APTES/nMo3Se4-rGO) was conducted onto hydrolysed indium tin oxide (ITO) coated glass platform by using electrophoretic deposition (EPD) technique at a DC potential of 15 V for 60 s. The APTES/nMo3Se4-rGO/ITO platform was then immobilized with monoclonal anti-cardiac troponin I (anti-cTnI) via EDC-NHS chemistry and bovine serum albumin (BSA) was employed for blocking of non-specific sites. The morphological and functional characterization of synthesized materials and fabricated electrodes were characterized by X-ray diffraction, Scanning electron microscopy, Transmission electron microscopy, Fourier transmission infrared spectroscopy and Atomic force microscopy. The electrochemical characterization and biosensing performance of the fabricated bioelectrodes was investigated by cyclic v...
Source: Microchemical Journal - Category: Chemistry Source Type: research