Viruses, Vol. 12, Pages 206: Quantitative FRET-FLIM-BlaM to Assess the Extent of HIV-1 Fusion in Live Cells

We present a new approach that employs fluorescence lifetime imaging microscopy (FLIM) to detect Förster resonance energy transfer (FRET) when using the β-lactamase (BlaM) assay. This novel approach allows comparing different populations of single cells regardless the concentration of CCF2-AM FRET reporter in each cell, and more importantly, is able to determine the relative amount of viruses internalized per cell. We have applied this approach in both reporter TZM-bl cells and primary T cell lymphocytes.

Source: Viruses - February 11, 2020 Category: Virology Authors: Carlon-Andres Padilla-Parra Tags: Article Source Type: research