Structural characterization and immunomodulatory activity of an exopolysaccharide produced by Lactobacillus helveticus LZ-R-5

Publication date: Available online 11 February 2020Source: Carbohydrate PolymersAuthor(s): Xiu You, Zhi Li, Kai Ma, Changliang Zhang, Xiaohong Chen, Guangxian Wang, Lin Yang, Mingsheng Dong, Xin Rui, Qiuqin Zhang, Wei LiAbstractExopolysaccharide (R-5-EPS) was isolated from the fermented milk of Lactobacillus helveticus LZ-R-5 and purified by DEAE-52 cellulose anion-exchange column, and characterization of the structure was conducted. Results showed that R-5-EPS was a heteropolysaccharide containing linear repeating units of →6)-β-D-Galp-(1→3)-β-D-Glcp-(1→3)-β-D-Glcp-(1→3)-β-D-Glcp-(1→3)-β-D-Glcp-(1→ with an average Mw of 5.41 × 105 Da. Furthermore, at a cellular level, R-5-EPS showed immunostimulatory activity due to its strong effect on increasing proliferation of RAW264.7 macrophages and enhancing phagocytosis, acid phosphatase activity, nitric oxide production and cytokines production in macrophages. These results suggest that R-5-EPS have a potent immunostimulatory activity and may be explored as a potential immunomodulatory agent.Graphical abstract
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research