Functional silica nanospheres for sensitive detection of H9N2 avian influenza virus based on immunomagnetic separation

Publication date: 1 May 2020Source: Sensors and Actuators B: Chemical, Volume 310Author(s): Fanwei Luo, Chao Long, Zhen Wu, Huayu Xiong, Miaomiao Chen, Xiuhua Zhang, Wei Wen, Shengfu WangAbstractElectrochemiluminescence (ECL) has been widely used in detection of biomarkers. However, the lacks of efficient ECL emitter and anti-interference ability limit its clinical application. Here, a sensitive ECL immunosensor was proposed for H9N2 avian influenza virus (H9N2 AIV) detection using functional silica nanospheres as signal carriers and magnetic nanobeads (MBs) as capture carriers. Functional silica nanospheres were prepared by encapsulating Ru(bpy)32+ in silica nanoparticles (RuSi NPs) followed by modifying with polyclonal antibody (pAb), amplifying the ECL signals by about 103-fold compared with the same concentration of Ru(bpy)32+. Targets could be directly captured and separated by MBs without sample pretreatment, which saved time and simplified operation. The integration of immunomagnetic separation improved the anti-interference ability, enabling the method feasible for practical applications. Based on functional silica nanospheres and MBs, the ECL immunosensor achieved ultrasensitive detection of 14 fg/mL H9N2 AIV with a dynamic range of 25 fg/mL to 25 ng/mL. Owing to its high sensitivity, good stability, selectivity, and anti-interference ability in complex samples through immunomagnetic separation, the method showed wonderful potential of application in clinical diagnos...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research