Both JNK1 and JNK2 Are Indispensable for Sensitized Extracellular Matrix Mineralization in IKK β-Deficient Osteoblasts

In this study, we used CRISPR/Cas9 system to permanently inactivate IKKβ in preosteoblast cells and confirmed that such cells displayed dramatic increase in extracellular matrix mineralization associated with JNK phosphorylation. Such observation was also found in our study using IKKβ-deficient primary murine osteoblasts. Interestingly, we found that in Ikbkb−/−Mapk8−/− or Ikbkb−/−Mapk9−/− double knockout cells, the enhanced mineralization caused by IKKβ deficiency was completely abolished, and deletion of either Mapk8 or Mapk9 was sufficient to dampen c-Jun phosphorylation. In further experiments, we discovered that absence of JNK1 or JNK2 on IKKβ-deficient background resulted in highly conserved transcriptomic alteration in response to osteogenic induction. Therefore, identification of the indispensable roles of JNK1 and JNK2 in activating c-Jun and promoting osteoblast differentiation on IKKβ-deficient background provided novel insights into restoring homeostasis in extracellular matrix mineralization.
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research

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Conclusions. Multiple factors were associated with a significantly increased risk of VFX compared with NVFX. Understanding the risk factors unique to fragility VFX is a critical component for targeting “at-risk” patients and preventing future osteoporosis-related fractures and their consequences. Level of Evidence: 4
Source: Spine - Category: Orthopaedics Tags: EPIDEMIOLOGY Source Type: research
An artificial-intelligence tool outperforms radiologists in flagging fractures on imaging and may help get more patients at risk for osteoporosis on appropriate therapy to prevent another fracture.Reuters Health Information
Source: Medscape Orthopaedics Headlines - Category: Orthopaedics Tags: Diabetes & Endocrinology News Source Type: news
AbstractPurpose of ReviewOpioids have been shown to be associated with an increased risk of fracture. The purpose of this paper is to review recent research into the effects of opioids on bone formation and bone healing in animal models and in human studies.Recent FindingsMost opioids, such as morphine and fentanyl, negatively affected bone remodeling and bone healing in animal models. Conversely, remifentanil has been recently shown to promote in vitro osteoblast differentiation and to inhibit differentiation and maturation of osteoclasts, therefore reducing bone resorption. According to the possible negative role of opio...
Source: Current Osteoporosis Reports - Category: Orthopaedics Source Type: research
AbstractPurpose of reviewThe main goal of this narrative review is to assess whether physical activity (PA) influences peak bone mass and fracture risk.Recent findingsSeveral randomized controlled trials (RCT) show that short-term PA intervention programs in childhood improve the accrual of bone mineral. There are now also long-term controlled PA intervention studies demonstrating that both boys and girls with daily school PA through puberty gain higher bone mineral content (BMC) and bone mineral density (BMD) and greater bone size than boys and girls with school PA 1 –2 times/week. These benefits seem to be followed...
Source: Current Osteoporosis Reports - Category: Orthopaedics Source Type: research
AbstractPurpose of ReviewThis review outlines the recent findings regarding the impact of bariatric surgery on bone. It explores potential mechanisms for skeletal changes following bariatric surgery and strategies for management.Recent FindingsBone loss following bariatric surgery is multifactorial. Probable mechanisms include skeletal unloading, abnormalities in calciotropic hormones, and changes in gut hormones. Skeletal changes that occur after bariatric surgery are specific to procedure type and persist for several years post-operatively. Studies suggest that while bone loss begins early, fracture risk may be increased...
Source: Current Osteoporosis Reports - Category: Orthopaedics Source Type: research
ConclusionThese findings demonstrate the ability of delivering Teriparatide orally using oleic acid based dispersion in combination with chitosan PEC.
Source: Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 5 April 2020Source: Journal of EthnopharmacologyAuthor(s): Eun-Young Kim, Jae-Hyun Kim, Minsun Kim, Jae Ho Park, Youngjoo Sohn, Hyuk-Sang Jung
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
This study delves into the mechanisms by which a short period of fasting can accelerate wound healing. Fasting triggers many of the same cellular stress responses, such as upregulated autophagy, as occur during the practice of calorie restriction. It isn't exactly the same, however, so it is always worth asking whether any specific biochemistry observed in either case does in fact occur in both situations. In particular, the period of refeeding following fasting appears to have beneficial effects that are distinct from those that occur while food is restricted. Multiple forms of therapeutic fasting have been repor...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
CONCLUSIONS: Our study showed that BTMs correlated negatively with lumbar BMD and positively with each other. Resorption markers levels increase with duration of estradiol deprivation period. PMID: 32239087 [PubMed - in process]
Source: Romanian Journal of Morphology and Embryology - Category: General Medicine Tags: Rom J Morphol Embryol Source Type: research
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Source: Endocrine Journal - Category: Endocrinology Tags: Endocr J Source Type: research
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