Cancers, Vol. 12, Pages 428: The Impact of Cand1 in Prostate Cancer

Cancers, Vol. 12, Pages 428: The Impact of Cand1 in Prostate Cancer Cancers doi: 10.3390/cancers12020428 Authors: Eigentler Tymoszuk Zwick Schmitz Pircher Kocher Schlicker Lesche Schäfer Theurl Klocker Heidegger Evidence has accumulated asserting the importance of cullin-RING (really interesting new gene) ubiquitin ligases (CRLs) and their regulator Cullin-associated neural-precursor-cell-expressed developmentally down-regulated 8 (NEDD8) dissociated protein 1 (Cand1) in various cancer entities. However, the role of Cand1 in prostate cancer (PCa) has not been intensively investigated so far. Thus, in the present study, we aimed to assess the relevance of Cand1 in the clinical and preclinical setting. Immunohistochemical analyses of radical prostatectomy specimens of PCa patients showed that Cand1 protein levels are elevated in PCa compared to benign areas. In addition, high Cand1 levels were associated with higher Gleason Scores, as well as higher tumor recurrence and decreased overall survival. In line with clinical findings, in vitro experiments in different PCa cell lines revealed that knockdown of Cand1 reduced cell viability and proliferation and increased apoptosis, therefore underlining its role in tumor progression. We also found that the cyclin-dependent kinase inhibitor p21 is significantly upregulated upon downregulation of Cand1. Using bioinformatic tools, we detected genes encoding for proteins linked to mRNA turnover, p...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research