Porcine heart valve, aorta and trachea cryopreservation and thawing using polydimethylsiloxane.

This study is aimed to improve a biological tissues cryopreservation by adding a new heat transfer fluid - polydimethylsiloxane (PDMS). To evaluate its efficiency the porcine biological tissues (heart valves, aortic and trachea fragments) were cryopreserved and thawed in low-viscous PDMS. According to the computer simulation, the midsection cooling rate was up to 490 °C/min and the midsection thawing rate was up to 1140 °C/min with admissible temperature uniformity. Cryoprotectants and liquid nitrogen were not used. The quality of tissue cryopreservation was evaluated using a number of histological and immunohistochemical methods (Orcein, H&E, Anti-CD34, Anti-Vimentin, Anti-Actin staining). Cryopreserved tissues showed no significant morphological difference in comparison with control group both in case of immediate thawing, and after 2 months of low temperature storage. Computer simulation of heat transfer showed the thermal limitations of used approach for larger specimens. The use of PDMS is proposed for preservation of vascular tissue in order to implant it in the form of homotransplants or biobanking with the possible additional use of an internal hydrophilic coating to prevent hydrophobization. PMID: 32045567 [PubMed - as supplied by publisher]
Source: Cryobiology - Category: Biology Authors: Tags: Cryobiology Source Type: research

Related Links:

ConclusionIn children, the results of mitral valve replacement were found to be inferior to those of mitral valve repair. The reoperation rates are similar in patients undergoing initial repair or replacement, which favors repair as an option. In developing world, rheumatic mitral valve disease is more prevalent where adequate facilities for monitoring of prosthetic valve function and management of anticoagulation therapy are not easily available. Valve repair therefore should be the primary goal.
Source: Indian Journal of Thoracic and Cardiovascular Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
Conclusion: The most frequent isolated valve surgery in Brazil is the aortic valve replacement by conventional open access and the rheumatic disease is still the main etiology for valve surgery. The BYPASS Registry has a fundamental role to provide information on the profile of patients with valve heart disease in our country in order to delineate adequate strategies for health promotion and resource allocation for cardiac surgery.
Source: Revista Brasileira de Cirurgia Cardiovascular - Category: Cardiovascular & Thoracic Surgery Source Type: research
In the field of prosthetic transcatheter heart valves (THV) it has become a tradition to compare outcomes of different generations within a device family. This approach is justified and necessary to demonstrate potential improvements of the successor models, but also potential deteriorations, as it is unlikely – and probably unethical – that anyone would conduct a randomized comparison. Transcatheter aortic valve implantation (TAVI) has evolved to a sophisticated procedure and has become a cornerstone in the treatment of severe aortic stenosis [1].
Source: International Journal of Cardiology - Category: Cardiology Authors: Tags: Editorial Source Type: research
AbstractFunctional tricuspid regurgitation (FTR) is defined as abnormal systolic tricuspid leakage with normal valve structures, and its prognostic role and management in patients with left-heart valve disease is well known. Due to paucity of data on FTR in patients with ischemic heart disease, the aim of our prospective study was to compare the prognostic effect of FTR between patients with moderate FTR and those with less-than-moderate FTR undergoing isolated coronary artery bypass graft (CABG) surgery. This prospective cohort study included all the patients who were candidate for isolated CABG and were referred for preo...
Source: The International Journal of Cardiovascular Imaging - Category: Radiology Source Type: research
RARITAN, N.J., March 20, 2020 – The Janssen Pharmaceutical Companies of Johnson &Johnson announced today that it will unveil late-breaking data from its leading cardiovascular and metabolism portfolio during the virtual American College of Cardiology’s 69th Annual Scientific Session together with the World Congress of Cardiology (ACC.20/WCC) on March 28-30, 2020. Notably, four late-breaking abstracts for XARELTO® (rivaroxaban) will be presented, including data from the Phase 3 VOYAGER PAD study in patients with symptomatic peripheral artery disease (PAD) after lower-extremity revascularization.Click to ...
Source: Johnson and Johnson - Category: Pharmaceuticals Source Type: news
Initial orientation of transcatheter heart valves may potentially preserve coronary access, a study suggests.Medscape Medical News
Source: Medscape Cardiology Headlines - Category: Cardiology Tags: Cardiology News Source Type: news
Abstract Recent advancements in 3D printing have revolutionized biomedical engineering by enabling the manufacture of complex and functional devices in a low-cost, customizable, and small-batch fabrication manner. Soft elastomers are particularly important for biomedical applications because they can provide similar mechanical properties as tissues with improved biocompatibility. However, there are very few biocompatible elastomers with 3D printability, and little is known about the material properties of biocompatible 3D printable elastomers. Here, we report a new framework to 3D print a soft, biocompatible, and ...
Source: Journal of the Mechanical Behavior of Biomedical Materials - Category: Materials Science Authors: Tags: J Mech Behav Biomed Mater Source Type: research
In this study we investigate the rate-dependency of the mechanical behaviour of semilunar heart valves under biaxial deformation, from quasi-static to physiological loading rates. This work extends and complements our previous undertaking, where the rate-dependency in the mechanical behaviour of semilunar valve specimens was documented in sub-physiological rate domains (Acta Biomater. 2019; https://doi.org/10.1016/j.actbio.2019.02.008). For the first time we demonstrate herein that the stress-stretch curves obtained from specimens under physiological rates too are markedly different to those at sufficiently lower rates and...
Source: Journal of the Mechanical Behavior of Biomedical Materials - Category: Materials Science Authors: Tags: J Mech Behav Biomed Mater Source Type: research
ConclusionsFollowing heart valve surgery, the risk of recurrent IE was significantly higher than the risk of first-time IE.Graphic abstract
Source: Clinical Research in Cardiology - Category: Cardiology Source Type: research
CONCLUSIONS: Bleeding and thromboembolic complications were common in MHV prosthesis patients in this study. Long duration of anticoagulation, albeit sub-optimal control, was a risk factor for bleeding and thromboembolic complications in these patients. Therefore, long-term efforts are necessary to address these complications and possibly improve the quality of life of these patients. PMID: 32181794 [PubMed - as supplied by publisher]
Source: Cardiovascular Journal of Africa - Category: Cardiology Authors: Tags: Cardiovasc J Afr Source Type: research
More News: Biology | Cardiology | Computers | Heart | Heart Valve Disease | Heart Valves | Study