High-complexity extracellular barcoding using a viral hemagglutinin [Microbiology]

While single-cell sequencing technologies have revealed tissue heterogeneity, resolving mixed cellular libraries into cellular clones is essential for many pooled screens and clonal lineage tracing. Fluorescent proteins are limited in number, while DNA barcodes can only be read after cell lysis. To overcome these limitations, we used influenza virus hemagglutinins...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Brief Reports Biological Sciences Source Type: research