Protective efficacy of recombinant bacterin vaccine against botulism in cattle.

In this study, the recombinant protein vaccine was produced using an Escherichia coli cell-based system. The formaldehyde-inactivated E. coli is able to induce 7.45 ± 1.77 and 6.6 ± 1.28 IU/mL neutralizing mean titers against BoNTs C and D in cattle, respectively, determined by mouse neutralization bioassay, and was deemed protective by the Brazilian legislation. Moreover, when the levels of anti-BoNT/C and D were compared with those achieved by the recombinant purified vaccines, no significant statistical difference was observed. Cattle vaccinated with the commercial vaccine developed 1.33 and 3.33 IU/mL neutralizing mean titers against BoNT serotypes C and D, respectively. To the best of our knowledge, this study is the first report on recombinant E. coli bacterin vaccine against botulism. The vaccine was safe and effective in generating protective antibodies and, thus, represents an industry-friendly alternative for the prevention of cattle botulism.
PMID: 32037222 [PubMed - as supplied by publisher]

Source: Vaccine - February 5, 2020 Category: Allergy & Immunology Authors: Moreira C, Ferreira MRA, Finger PF, Magalhães CG, Cunha CEP, Rodrigues RR, Otaka DY, Galvão CC, Salvarani FM, Moreira ÂN, Conceição FR Tags: Vaccine Source Type: research