Source: Human Vaccines and Immunotherapeutics - Category: Allergy & Immunology Authors: Pasquale Stefanizzi Sara De Nitto Francesco Patano Francesco Paolo Bianchi Davide Ferorelli Paolo Stella Domenica Ancona Vito Bavaro Silvio TafuriSource Type: research
Immunity to measles, mumps, rubella, and varicella-zoster viruses (VZV; MMRV) is a common condition of employment for health care workers (HCWs) to ensure compliance with national standards and state laws. When documentation of complete vaccination or laboratory-confirmed infection is not available, Advisory Committee on Immunization Practices (ACIP) criteria are used to guide vaccination or anti-MMRV IgG testing. We assessed the performance of the BioPlex 2200 MMRV IgG multiplex flow immunoassay (MFI; Bio-Rad Laboratories, Hercules, CA) and matched immunofluorescence assays (IFAs; MBL Bion, Des Plaines, IL) in 220 HCWs ca...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Theel, E. S., Sorenson, M., Rahman, C., Granger, D., Vaughn, A., Breeher, L.Tags: Immunoassays Source Type: research
Source: Human Vaccines and Immunotherapeutics - Category: Allergy & Immunology Authors: Paolo Bonanni Sara Boccalini Angela Bechini Ornella Varone Giulio Matteo Federica Sandri Giovanni GabuttiSource Type: research
Most commercially available enzyme immunoassay-based methods have limited sensitivity to detect antibody responses to varicella-zoster virus (VZV) in vaccinated individuals, who produce lower antibody levels than those with natural infection. However, more sensitive methods are either not commercially available or less amenable to high-throughput testing. The BioPlex 2200 measles, mumps, rubella, and varicella (MMRV) IgG assay (Bio-Rad Laboratories, Hercules, CA) is an automated high-throughput platform based on the microsphere Luminex technology that measures antibodies against measles, mumps, rubella, and varicella virus...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: McLachlan, E., Scholz, H., Bolotin, S., Crowcroft, N. S., Hatchette, T. F., Jackson, C., Severini, A., on behalf of the Immunity of Canadians and Risk of Epidemics (iCARE) Laboratory Group and the Canadian Immunization Research Network (CIRN)Tags: Immunoassays Source Type: research
CONCLUSION: The demonstration of batch consistency and non-inferiority of the Bio-MMR vaccine completed the technology transfer. This is a significant technological achievement with implications for immunisation programs.
PMID: 30843921 [PubMed - in process]
Source: Memorias do Instituto Oswaldo Cruz - Category: Infectious Diseases Authors: Santos EMD, Noronha TG, Alves IS, Cruz RLS, Ferroco CLV, Brum RC, Oliveira PMN, Siqueira MM, Lima MC, Ramos FLP, Bragagnolo CM, Camacho LAB, Maia MLSTags: Mem Inst Oswaldo Cruz Source Type: research
CONCLUSION: Demonstration of early disappearance of maternal antibodies against MMRV viruses leading to the risk of these infections at an early age emphasize need for early immunization of Indian children. Suboptimal response to measles vaccine needs to be seriously addressed especially in view of the WHO's initiative for measles eradication.
PMID: 30765170 [PubMed - as supplied by publisher]