Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay.

We examined proofreading of DNAs containing indel errors at various positions of the primer-template junction. We found that indel errors located 1-5-nucleotides (nt) from the primer terminus can be proofread efficiently, while insertion/deletions at 6-nt from the 3' end are partially corrected and extended. Indels located 7-9-nt from the primer terminus escape proofreading and are elongated by polymerase. The possible underlying mechanisms of these observations are discussed in the context of the polymerase and primer-template junction interactions via a structure analysis.
PMID: 32036259 [PubMed - as supplied by publisher]

Source: DNA Repair - January 29, 2020 Category: Genetics & Stem Cells Authors: Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH Tags: DNA Repair (Amst) Source Type: research