Optimization of the collagen extraction from Nile tilapia skin (Oreochromis niloticus) and its hydrogel with hyaluronic acid

Publication date: Available online 8 February 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Maria do L.L.R. Menezes, Hálisson L. Ribeiro, Flávia de Oliveira M.S. Abreu, Judith P.A. Feitosa, Men de Sá Moreira de S. FilhoAbstractNile tilapia skin, an abundant waste from fish processing, can be used for collagen extraction, which has a high aggregated value for biomedical applications. Collagen extraction was conducted under different reaction conditions (time, temperature, and concentration of acetic acid) in order to optimize the yield without compromising the integrity of the collagen. Temperature and time were responsible for increased yield. The extraction at 4 and 20 °C produced the acid-solubilized collagen (ASC) with the intact triple helix and was analysed by Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism (CD). The optimized ASC (which used 0.35 mol/L of acetic acid at 20 °C) was consumed to obtain for the first-time fish-based hydrogels with hyaluronic acid (HA) crosslinked with 1-ethyl-3-(3-dimethylaminopropryl carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The hydrogel was characterized by FT-IR, rheology, swelling, and scanning electron microscopy (SEM), confirming that cross-linking was accomplished. It possesses a robust organized network, swells 255% in PBS and bears interconnected pores with a diameter in the range of 10-100 µm. Until now, col-HA hydrogels crosslinked with EDC/NHS have not been reported in...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research