Inactivation of foot-and-mouth disease virus A/IRN/8/2015 with commercially available lysis buffers.

In this study, three commercial lysis buffers (AL, AVL, and MagMAX CORE) were tested in two laboratories for their ability to inactivate FMDV A/IRN/8/2015 in different sample matrices (cell culture supernatant, epithelial tissue suspension and milk). Residual infectivity after the addition of lysis buffer was evaluated by inoculating susceptible cell cultures. No cytopathic effect was observed for all three lysis buffers, indicating that the buffers are capable of reducing viral infectivity (estimated range 3.1 to >5.1 Log10). These results highlight the capacity of lysis buffers to decrease FMDV infectivity; however, additional validation experiments should be conducted, particularly if different sample matrices and/or lysis buffers are used.
PMID: 32035122 [PubMed - as supplied by publisher]

Source: Journal of Virological Methods - February 4, 2020 Category: Virology Authors: Wood BA, Mioulet V, Henry E, Gray A, Azhar M, Thapa B, Diederich S, Hoffmann B, Beer M, King DP, Eschbaumer M Tags: J Virol Methods Source Type: research