Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics

Publication date: Available online 7 February 2020Source: Redox BiologyAuthor(s): Ana Isabel Santos, Ana Sofia Lourenço, Sónia Simão, Dorinda Marques da Silva, Daniela Filipa Santos, Ana P. Onofre de Carvalho, Ana Catarina Pereira, Alicia Izquierdo-Álvarez, Elena Ramos, Esperanza Morato, Anabel Marina, Antonio Martínez-Ruiz, Inês M. AraújoAbstractNitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R–SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the prol...
Source: Redox Biology - Category: Biology Source Type: research