Elucidation of the distal convoluted tubule transcriptome identifies new candidate genes involved in renal magnesium handling.

Elucidation of the distal convoluted tubule transcriptome identifies new candidate genes involved in renal magnesium handling. Am J Physiol Renal Physiol. 2013 Oct 2; Authors: de Baaij JH, Groot Koerkamp MJ, Lavrijsen M, van Zeeland F, Meijer H, Holstege FC, Bindels RJ, Hoenderop JG Abstract The kidney plays a key role in the maintenance of the magnesium (Mg(2+)) homeostasis. Specifically, the distal convoluted tubule (DCT) is instrumental in fine-tuning of the renal Mg(2+) handling. In recent years hereditary Mg(2+) transport disorders have helped to identify important players in DCT Mg(2+) homeostasis. Nevertheless, several proteins involved in DCT-mediated Mg(2+) reabsorption remains to be discovered and a full expression profile of this complex nephron segment may facilitate the discovery of new Mg(2+)-related genes. Here, we report the Mg(2+)-sensitive expression of the DCT transcriptome. To this end, transgenic mice expressing eGFP under a DCT-specific parvalbumin promoter were subjected to Mg(2+)-deficient or Mg(2+)-enriched diets. Subsequently, the Complex Object Parametric Analyzer and Sorter (COPAS) allowed for the first time isolation of eGFP-positive DCT cells. RNA extracts thereof were analyzed by DNA microarrays comparing high vs. low Mg(2+) to identify Mg(2+) regulatory genes. Based on statistical significance and a fold-change of at least two, 46 genes showed differential expression. Several known magnesiotropic genes, such as ...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research