Understanding mass spectrometry based global mycobacterial lipidomics.

Understanding mass spectrometry based global mycobacterial lipidomics. Curr Mol Med. 2020 Feb 06;: Authors: Fatima Z, Nandan S, Hameed S Abstract Tuberculosis (TB) is the foremost cause of mortality from single infectious agent Mycobacterium tuberculosis (MTB). Current therapeutic regimes suffer from several problems including side effects, costs and emergence of multidrug resistance (MDR). Moreover, conventional diagnostic methods are either too slow, lack accurate or robust biomarkers. Under such circumstances, identification of rapid metabolite based biomarkers as novel drug targets could be a potential approach to circumvent MDR. In the era of "OMIC" sciences, lipidomics has gain significant attention to unravel the complexity of lipid loaded Mycobacterium species. Lipidomics is a sub-branch of metabolomics with extreme atomic diversity between the metabolites. There is no single principle on which the metabolite diversity can be defined, unlike other biomolecules viz. nucleic acid, proteins or carbohydrates. MTB encodes 10% of the genome for lipid metabolism and lipids accounts for 60% of its dry weight. Mycobacterium harbor wide spectra of lipid repertoire ranging from highly apolar to highly polar lipids adding the complexity in their identification and analysis. Compared to targeted approaches, untargeted or global lipidomics of MTB is still more challenging. This review describes recent advances in lipidomic technology with ...
Source: Current Molecular Medicine - Category: Molecular Biology Authors: Tags: Curr Mol Med Source Type: research