Preparation and optimization of fisetin loaded glycerol based soft nanovesicles by Box-Behnken Design

Publication date: Available online 6 February 2020Source: International Journal of PharmaceuticsAuthor(s): Thasleem Moolakkadath, Mohd. Aqil, Abdul Ahad, Syed Sarim Imam, interpreting the data, Arshiya Praveen, Yasmin Sultana, Mohd MujeebAbstractThe present study focused on the development and optimization of glycerosomes for dermal delivery of fisetin. The fisetin loaded glycerosomes formulation was optimized by Box-Behnken design. The independent variables were the lipoid S 100, glycerol, and sonication time, whereas the dependent variables were the vesicles size, entrapment efficiency, and flux. The mechanism of skin penetration of fisetin loaded glycerosomes formulation was determined by the DSC and FTIR studies. Confocal scanning microscopy was used to detect the penetration ability of glycerosomes. The optimized fisetin loaded glycerosomes formulation was converted into a Carbopol® gel matrix, and the latter was analyzed for various parameters. The optimized formulation of glycerosomes presented vesicles size, entrapment efficiency and flux of 138.8 ± 4.09 nm, 86.41 ± 2.95% and 5.04 ± 0.17 µg/cm2/h, respectively. The transmission electron microscopy of optimized fisetin loaded formulation revealed the spherical and sealed structure of glycerosomes vesicles. The confocal study confirmed that the Rhodamine B incorporated glycerosomes penetrated up to deeper layers of skin. The DSC and FTIR studies revealed that the hydration of skin layers and skin lipid fluidization...

Source: International Journal of Pharmaceutics - February 7, 2020 Category: Drugs & Pharmacology Source Type: research