Long-term (24h) cooling of ovarian fragments in the presence of permeable cryoprotectants prior to freezing: Spontaneous pregnancy and baby born after re-transplantation.

Long-term (24h) cooling of ovarian fragments in the presence of permeable cryoprotectants prior to freezing: Spontaneous pregnancy and baby born after re-transplantation. Cryobiology. 2020 Jan 31;: Authors: Isachenko V, Morgenstern B, Todorov P, Isachenko E, Mallmann P, Hanstein B, Rahimi G Abstract Cancer is the second major cause of death in the world. The problem of post-cancer infertility plays a significant role, because chemotherapy can be gonadotoxic. Cryopreservation of ovarian tissue before cancer therapy with re-implantation after convalescence is the potential key solution to this problem. The aim of this study was to test the viability of cryopreserved human ovarian cortex after long-term cooling in culture medium composed of permeable cryoprotectants. Ovarian fragments from sixteen patients were randomly divided into two groups. After the operation, tissue pieces assigned to both groups were cooled to 5 °C for 22-24 h, frozen and thawed. Group 1 pieces (n = 32) were cooled before cryopreservation in the standard culture medium, and Group 2 pieces (n = 32) were cooled in the freezing medium (culture medium+6% ethylene glycol+6% dimethyl sulfoxide+0.15 M sucrose). Freezing was performed in standard 5 ml cryo-vials with ice formation at -9 °C, cooling from -9 to -34 °C at a rate of -0.3 °C/min and plunging at -34 °C into liquid nitrogen. Thawing was performed in a 1...
Source: Cryobiology - Category: Biology Authors: Tags: Cryobiology Source Type: research

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Editor’s Note: For more on the challenges as well as strategies to address physician infertility, please read this Academic Medicine Invited Commentary, cowritten by the author of this blog post. Most of us in medicine pride ourselves on being organized, detail-oriented, conscientious and able to achieve excellent results with enough hard work—and are not used to failure and loss of control. My experience with infertility has been the first time that I truly experienced failure and complete loss of control, realizing that fertility is not a merit-based system. No matter how hard I try, how many do...
Source: Academic Medicine Blog - Category: Universities & Medical Training Authors: Tags: Featured Guest Perspective infertility physician well-being women in academic medicine Source Type: blogs
Abstract Breast cancer (BC) is the most common malignancy in women with a significant increasing incidence during the reproductive life. However, based on the newest anti-cancer molecular targeting drugs, successful treatments lead to the disease healing particularly in young patients, thus refreshing their motherhood programs. However, as effect of the BC treatment, a premature depletion of the ovarian follicle reserve occurs in more than one-third of patients resulting in permanent infertility. To prevent the cancer treatment-related infertility (CTRI), several options are today utilized. Besides the ovary suppr...
Source: Clinical Breast Cancer - Category: Cancer & Oncology Authors: Tags: J Cancer Res Clin Oncol Source Type: research
Authors: Lee D, Kim SK, Lee JR, Jee BC Abstract Endometriosis is a common inflammatory disease in women of reproductive age and is one of the major causes of infertility. Endometriosis causes a sustained reduction of ovarian reserve through both physical mechanisms and inflammatory reactions, which result in the production of reactive oxygen species and tissue fibrosis. The severity of endometriosis is related to ovarian reserve. With regard to infertility treatment, medical therapy as a neoadjuvant or adjuvant to surgical therapy has no definite beneficial effect. Surgical treatment of endometriosis can lead to ov...
Source: Clinical and Experimental Reproductive Medicine - Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research
AbstractBreast cancer (BC) is the most common malignancy in women with a significant increasing incidence during the reproductive life. However, based on the newest anti-cancer molecular targeting drugs, successful treatments lead to the disease healing particularly in young patients, thus refreshing their motherhood programs. However, as effect of the BC treatment, a premature depletion of the ovarian follicle reserve occurs in more than one-third of patients resulting in permanent infertility. To prevent the cancer treatment-related infertility (CTRI), several options are today utilized. Besides the ovary suppression by ...
Source: Journal of Cancer Research and Clinical Oncology - Category: Cancer & Oncology Source Type: research
AbstractPurpose of ReviewYoung women represent a minority of breast cancer patients for which fertility, family planning, and pregnancy represent unique vulnerabilities. This review intends to discuss recent published evidence regarding treatment-related infertility, fertility counseling, and preservation.Recent FindingsFertility concerns are common among young women with breast cancer and may negatively affect treatment decisions. Data is available to aid providers in approximating odds of post-treatment amenorrhea and infertility. Multiple fertility preservation techniques are available. While embryo preservation is most...
Source: Current Breast Cancer Reports - Category: Cancer & Oncology Source Type: research
The objective of this narrative review was to critically evaluate the available literature regarding the capacity for the uterus to sustain a healthy pregnancy following exposure to radiotherapy or chemotherapy.SEARCH METHODSThe authors performed PubMed (Medline) searches using the following key words: uterus, cancer survivors, radiotherapy, chemotherapy, pregnancy outcome, fertility preservation, infertility. There were no limits placed on time of publication.OUTCOMESOverall, there were major limitations to the current available literature, meaning that interpretations should be taken with caution. Despite these drawbacks...
Source: Human Reproduction Update - Category: OBGYN Source Type: research
Rational: The growing population of young cancer survivors and a trend toward postponing pregnancy until later years in life are leading to a deeper attention towards understanding treatment-induced sequelae, and, in particular, the effects of cancer and/or treatment on fertility. Nowadays, the infertility risks potentially associated with molecular targeted therapies are not established, and clinical reports are sparse. Moreover, the increasing use of molecular targeted drugs in the adjuvant setting and in diseases with better prognosis makes preservation of fertility a major topic in current research. Patient's concer...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Clinical Case Report Source Type: research
ConclusionsWomen with an early stage of breast cancer and a possible future child wish should be referred to an expertise center in breast cancer, fertility preservation, and genetics in this complex decision-making process, shortly after diagnosis.
Source: Journal of Assisted Reproduction and Genetics - Category: Reproduction Medicine Source Type: research
Purpose of review To investigate the JZE alterations in gynecological and obstetrical disorders and impact on diagnosis, prognosis and treatment. Recent findings JZE was found to be significantly extended in patients with endometriosis, leading to the conclusion that endometriosis is a primary disease of the uterus, much like adenomyosis. Statistical correlation was then demonstrated between the severity of endometriosis and the depth of the adenomyosis infiltrates, hence the thickening of the JZE. Stem cells, predominantly found in the JZE were also found in histological sections of leiomyoma, suggested to be the ori...
Source: Current Opinion in Obstetrics and Gynecology - Category: OBGYN Tags: WOMEN'S HEALTH: Edited by Joseph Aquilina Source Type: research
ConclusionEndometrial cancer, specifically type I endometrioid adenocarcinoma and its precursor lesions are strongly associated with unopposed estrogen states such as polycystic ovarian syndrome (PCOS), obesity as well as nulliparity. Patients seeking assisted reproductive technologies make up a subset of these patients and strongly desire to maintain their fertility. Progesterone therapy for select patients may be a safe and effective temporary treatment for endometrial carcinoma and atypical precursor lesions prior to starting IVF-ET. IVF-ET is very effective in treating such patients.
Source: Journal of Minimally Invasive Gynecology - Category: OBGYN Source Type: research
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