Long-term (24h) cooling of ovarian fragments in the presence of permeable cryoprotectants prior to freezing: Spontaneous pregnancy and baby born after re-transplantation.

Long-term (24h) cooling of ovarian fragments in the presence of permeable cryoprotectants prior to freezing: Spontaneous pregnancy and baby born after re-transplantation. Cryobiology. 2020 Jan 31;: Authors: Isachenko V, Morgenstern B, Todorov P, Isachenko E, Mallmann P, Hanstein B, Rahimi G Abstract Cancer is the second major cause of death in the world. The problem of post-cancer infertility plays a significant role, because chemotherapy can be gonadotoxic. Cryopreservation of ovarian tissue before cancer therapy with re-implantation after convalescence is the potential key solution to this problem. The aim of this study was to test the viability of cryopreserved human ovarian cortex after long-term cooling in culture medium composed of permeable cryoprotectants. Ovarian fragments from sixteen patients were randomly divided into two groups. After the operation, tissue pieces assigned to both groups were cooled to 5 °C for 22-24 h, frozen and thawed. Group 1 pieces (n = 32) were cooled before cryopreservation in the standard culture medium, and Group 2 pieces (n = 32) were cooled in the freezing medium (culture medium+6% ethylene glycol+6% dimethyl sulfoxide+0.15 M sucrose). Freezing was performed in standard 5 ml cryo-vials with ice formation at -9 °C, cooling from -9 to -34 °C at a rate of -0.3 °C/min and plunging at -34 °C into liquid nitrogen. Thawing was performed in a 100 °C (boiling) water bath. The removal ...
Source: Cryobiology - Category: Biology Authors: Tags: Cryobiology Source Type: research