Dynamics of the within-herd prevalence of Mycoplasma bovis intramammary infection in endemically infected dairy herds

Publication date: Available online 6 February 2020Source: Veterinary MicrobiologyAuthor(s): Anri A.E. Timonen, Tiina Autio, Tarja Pohjanvirta, Liidia Häkkinen, Jørgen Katholm, Anders Petersen, Kerli Mõtus, Piret KalmusAbstractWe aimed to identify the dynamics of the within-herd prevalence of Mycoplasma (M.) bovis intramammary infection (IMI) in four dairy herds, estimate prevalence of M. bovis in colostrum and clinical mastitis cases and compare M. bovis strains from calves’ respiratory and cow clinical mastitis samples. Within a six-month study period, cow composite milk samples (CMS) were collected three times during routine milk recording, first milking colostrum samples from all calving cows and udder quarter milk samples from clinical mastitis cases. Calf respiratory samples were collected from calves with respiratory disease. Pooled milk samples were analysed for M. bovis with the Mastitis 4B polymerase chain reaction (PCR) test kit (DNA Diagnostic A/S). Prevalence estimates were calculated with Bayesian framework in R statistical programme. cg-MLST was used for M. bovis genotyping. In Herd I and II first testing M. bovis IMI within-herd prevalence (95% credibility interval (CI)) was 4.7% (2.9; 6.8) and 3.4% (2.3; 4.6), changing to 1.0% (0.1; 1.7) and 0.8% (0.1; 1.4) in Herd I and 0.4% (0.0; 0.7) in Herd II at the next samplings. In Herd III and IV first testing M. bovis IMI within-herd prevalence was 12.3% (9.7; 15.2) and 7.8% (6.2; 9.5), changing to 4.6% (3.0; 6....
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research