Haemonchus contortus transthyretin domain - containing protein (HcTTR): a promising vaccine candidate against Haemonchus contortus infection

Publication date: Available online 5 February 2020Source: Veterinary ParasitologyAuthor(s): Xiaowei Tian, Mingmin Lu, Caiwen Jia, Yongqian Bu, Kalibixiati Aimulajiang, Yang Zhang, Charles Li, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xiangrui LiAbstractHaemonchus contortus transthyretin domain-containing protein (HcTTR) with 136 amino acids belongs to a transthyretin-like (TTL) family member. In our previous study, it was reported that HcTTR was a novel antagonist of the goat cytokine Interleukin 4 (IL-4), and was involved in the regulation of host immune responses, implying that it might be applied for therapeutic strategies or vaccine development against the infection of H. contortus. Thus, the protective capacities of HcTTR against H. contortus infections via active and passive immunization trials were examined. For the passive protection trials, H. contortus-infected goats were intravenously immunized twice with 5 mg of total IgG containing anti-rHcTTR goat polyclonal antibodies. The results showed that the significant rates of reduction in egg shedding and worm burden was 58.12% and 64.61%, respectively, as compared with the positive control group. For the active protection trials, local goats were vaccinated twice with 500 µg of recombinant HcTTR to generate antigen-specific circulating antibodies, resulting in 63.7% reduction in eggs shedding and 66.4% reduction in worm burdens after H. contortus challenge. In both passive and active trials, the immunized goats display...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research