The role of profilin-1 in endothelial cell injury induced by advanced glycation end products (AGEs)

This study aimed to investigate the pivotal role of profilin-1 in the process of endothelial cell damage induced by AGEs. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with AGEs. The mRNA and protein expression of profilin-1 was determined using real-time PCR and western blotting analyses. The levels of intercellular adhesion molecule-1 (ICAM-1), nitric oxide (NO) and reactive oxygen species (ROS), as well as the activities of nuclear factor-kappaB (NF-kappaB) and protein kinase C (PKC), were detected using the appropriate kits. The levels of asymmetric dimethylarginine (ADMA) were determined using HPLC. The distribution of the cytoskeleton was visualised using immunofluorescent staining. Results: Compared with the control, incubation of endothelial cells with AGEs (200 mug/ml) for 4 or 24 h significantly up-regulated the mRNA and protein expression of profilin-1, markedly increased the levels of ICAM-1 and ADMA and decreased the production of NO (P
Source: Cardiovascular Diabetology - Category: Cardiology Authors: Source Type: research