Evaluation of Bronchodilator and antimicrobial activities of Otostegia fruticose: A multi-mechanistic approach

Publication date: Available online 31 January 2020Source: Saudi Pharmaceutical JournalAuthor(s): Mohd Nazam Ansari, Najeeb-ur Rehman, Aman Karim, Tiegsti Bahta, Khalil Y. Abujheisha, Syed Rizwan Ahamad, Faisal ImamAbstractOtostegia fruticose, a plant belonging to the family Lamiaceae, is endemic to Ethiopia. In Ethiopian traditional medicine, O. fruticose has been used for the treatment of several respiratory-related disorders. The present study was designed to evaluate the bronchodilator and antimicrobial activities of O. fruticose leaves crude extract (Of.Cr). Ex-vivo experiments were conducted on guinea-pig trachea provided with physiological oxygenated buffer solution using emkaBath setup. The crude extract was analyzed by gas chromatography-mass spectrometry. Of.Cr, showed the presence of terpenes, fragrance components, saponins, and higher fatty acids. Of.Cr when tested on contracted tracheal chains with carbamylcholine (CCh, 1 µM) and high K+ (80 mM) produced relaxation by showing higher potency against CCh with incomplete inhibition of high K+. Dicyclomine, used as a positive control, also showed selectively higher potency to inhibit CCh when compared with its effect against K+. In the anticholinergic curves, Of.Cr at 1 mg/mL deflected CCh-induced concentration-response curves (CRCs) competitively to the right like dicyclomine (0.03 µM) and atropine whereas a higher dose of Of.Cr (3 mg/mL) produced a non-parallel shift in the CCh curves like a higher dose of dicyclo...
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research