Rapid and low-cost quantitative detection of creatinine in human urine with a portable Raman spectrometer

Publication date: Available online 31 January 2020Source: Biosensors and BioelectronicsAuthor(s): Wei Zhu, Bao-Ying Wen, Ling-Jun Jie, Xiang-Dong Tian, Zhi-Lin Yang, Petar M. Radjenovic, Shi-Yi Luo, Zhong-Qun Tian, Jian-Feng LiAbstractThe creatinine concentration of human urine is closely related to human kidney health and its rapid, quantitative, and low-cost detection has always been demanded. Herein, a surface-enhanced Raman spectroscopic (SERS) method for rapid and cost-effective quantification of creatinine concentrations in human urine was developed. A Au nanoparticle solution (Au sol) was used as a SERS substrate and the influence of different agglomerating salts on its sensitivity toward detecting creatinine concentrations was studied and optimized, as well as the effect of both the salt and Au sol concentrations. The variation in creatinine spectra over time on different substrates was also examined, demonstrating reproducible quantitative analysis of creatinine concentrations in solution. By adjusting the pH, a simple liquid−liquid solvent extraction procedure, which extracted creatinine from human urine, was used to increase the SERS detection selectivity toward creatinine in complex matrices. The quantitative results were compared to those obtained with a clinically validated enzymatic “creatinine kit (CK).” The limit of detection (LOD) for the SERS technique was 1.45 mg L−1, compared with 3.4 mg L−1 for the CK method. Furthermore, cross-comparin...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research