Comprehensive study on the effects of total monomers' content and polymerization temperature control on the formation of the polymer-layer in preparation of insulin-imprinted magnetic nanoparticles

Publication date: Available online 1 February 2020Source: European Polymer JournalAuthor(s): Farnaz Goudarzi, Parisa HejaziAbstractThe potential of surface-modified magnetic nanoparticles was used as the support for molecular imprinting to separate insulin. The effects of the total monomers' content (at seven levels) and overall temperature (ambient, controlled at 23 and 28°C) during the formation of the surface polymer layer in aqueous media were for the first time explored via general factorial experimental design. Methacrylic acid (MAA) and acrylamide (AAm) were chosen as the functional monomers, and methylenebisacrylamide (MBAAm) was applied as the cross-linker. Six responses were simultaneously studied using the desirability function. The software results demonstrated the importance of choosing proper amounts of constituents and controlling the temperature. The optimum conditions were determined to be for the magnetic molecularly imprinted polymers synthesized at 23°C and with the molar ratio of 1:40:200:10 for Insulin:MAA:AAm:MBAAm with an imprinting factor of 2.5. TGA and VSM analyses confirmed the thin (about 7% of total weight) and uniform core-shell structure of polymers with high saturation magnetization (20.1 emu g−1). The outcomes of adsorption kinetics and isotherm revealed that the imprinted cavities located on or close to its surface, and are practical for the separation of low and high abundance of insulin. This system could be reused for at least five ti...
Source: European Polymer Journal - Category: Chemistry Source Type: research