Preanalytical Variables for the Genomic Assessment of the Cellular and Acellular Fractions of the Liquid Biopsy in a Cohort of Breast Cancer Patients
Liquid biopsy allows assessment of multiple analytes, providing temporal information with potential for improving understanding of cancer evolution and clinical management of patients. Although liquid biopsies are intensely investigated for prediction and response monitoring, preanalytic variables are of primary concern for clinical implementation, including categories of collection method and sample storage. Herein, an integrated high-density single-cell assay workflow for morphometric and genomic analysis of the liquid biopsy is used to characterize the effects of preanalytical variation and reproducibility of data from a breast cancer cohort.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Stephanie N. Shishido, Lisa Welter, Mariam Rodriguez-Lee, Anand Kolatkar, Liya Xu, Carmen Ruiz, Anna S. Gerdtsson, Sara Restrepo-Vassalli, Anders Carlsson, Joe Larsen, Emily J. Greenspan, E. Shelley Hwang, Kathryn R. Waitman, Jorge Nieva, Kelly Bethel, Ja Tags: Regular article Source Type: research