A multiplex PCR assay with a common primer for the  detection of eleven foodborne pathogens.

A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens. J Food Sci. 2020 Jan 30;: Authors: Tao J, Liu W, Ding W, Han R, Shen Q, Xia Y, Zhang Y, Sun W Abstract Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time-consuming and labor-intensive. Multiplex PCR technology, which can detect multiple targets in a single tube, has been increasingly applied to microbial detection due to its high specificity, sensitivity, and fast response. This paper is to establish a multiplex PCR technology mediated by a common primer for the detection of these 11 common foodborne pathogens in order to achieve the goal of nondirectional screening for these 11 common foodborne pathogens. The specificity of the established CP-MPCR detection system was first verified by 100 clinical isolates. The sensitivity of the CP-MPCR detection system was then detected by using cultured bacteria preparations and has been confirmed with a high sensitivity of 103 to 104 CFU/mL, among them, the sensitivity of the CP-MPCR for Vibrio cholerae and S. flexneri can even achieve 102 CFU/mL. Sixty anal swab samples collected from Suzhou CDC and 16 ...
Source: Anal Sci - Category: Chemistry Authors: Tags: J Food Sci Source Type: research

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CONCLUSIONS: Xyloglucan/gelose plus ORS was effective and safe in treating acute diarrhea in children. PMID: 33028102 [PubMed - as supplied by publisher]
Source: Expert Review of Gastroenterology and Hepatology - Category: Gastroenterology Tags: Expert Rev Gastroenterol Hepatol Source Type: research
Conclusions: (1) Data at our center suggests at least a regional implementation gap in GBS screening and IAP. (2) The decline in the resistance rate ofE. coli for all antimicrobial substances might indicate that the reduction of prenatal antibiotics use is beneficial and that neonatal antibiotic stewardship programs should include pregnant women as well.What is Known:• GBS screening and intrapartum antibiotic prophylaxis led to a 32%-reduction in GBS disease in Germany with a 0.75 (92:122) ratio of early-onset disease to late-onset disease in 2009–2010.• Prenatal antibiotic use might increase the risk of E....
Source: European Journal of Pediatrics - Category: Pediatrics Source Type: research
This study investigated antibacterial-producing Streptomyces isolated from the gut of estuarine fish Chanos chanos, emphasizing screening for the producer of peptide-containing antibacterial compounds. Eighteen isolates were found during preliminary screening, in which four isolates showed the best antibacterial activities. Based on the morphological, physiological, and biochemical characterization, as well as 16S rRNA partial sequencing, all of the four isolates belonged to Streptomyces. Three isolates were suspected as novel isolate candidates based on homology presentations and phylogenetic tree analysis. Disk-diffusion...
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
Publication date: Available online 9 October 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Cezhi Du, Chengyong Wang, Jianbo Sui, Lijuan Zheng
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
CONCLUSION: There is a high prevalence of MRSA causing abscesses in the Hispanic population evaluated in an ED in Puerto Rico. Systemic antibiotic use for the treatment of skin abscesses after incision and drainage remains high despite published guidelines arguing against their widespread use. Clindamycin resistance in our patient population appears to be more frequent than previously reported. PMID: 33031694 [PubMed - as supplied by publisher]
Source: Puerto Rico Health Sciences Journal - Category: International Medicine & Public Health Tags: P R Health Sci J Source Type: research
Publication date: December 2020Source: Microbial Pathogenesis, Volume 149Author(s): Cemile Selin Aksoy, Fatma Gizem Avci, Osman Mutluhan Ugurel, Basak Atas, Nihat Alpagu Sayar, Berna Sariyar Akbulut
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: Available online 9 October 2020Source: Journal of Global Antimicrobial ResistanceAuthor(s): Elma L. Leite, Wydemberg J. Araújo, Tatiana R. Vieira, Karoline S. Zenato, Priscylla C. Vasconcelos, Samuel Cibulski, Patricia E.N. Givisiez, Marisa R.I. Cardoso, Celso J.B. Oliveira
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
This study aims to analyze the splicing characteristics of dystrophin PEs and compare them with those of dystrophin canonical exons (CEs). Forty-two reported dystrophin PEs were divided into a splice site (ss) group and a splicing regulatory element (SRE) group. Five dystrophin PEs with characteristics of poison exons were identified and categorized as the possible poison exon group. The comparative analysis of each essential splicing signal among different groups of dystrophin PEs and dystrophin CEs revealed that the possible poison exon group had a stronger 3′ ss compared to any other group. As for auxiliary SR...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research
Publication date: Available online 8 October 2020Source: Journal of Clinical and Experimental HepatologyAuthor(s): Ravi Mohanka, Prashantha Rao, Mitul Shah, Amit Gupte, Vinayak Nikam, Mihir Vohra, Ruhi Kohli, Anurag Shrimal, Ankush Golhar, Ameya Panchwagh, Saurabh Kamath, Akash Shukla, Priyesh Patel, Somnath Chattopadhyay, Gaurav Chaubal, Yasmin Shaikh, Vidhi Dedhia, Shivali S. Sarmalkar, Ravikiran Maghade, Kavita Shinde
Source: Journal of Clinical and Experimental Hepatology - Category: Gastroenterology Source Type: research
Conclusion: The incidence of visual dysfunction in patients with CO poisoning was high, the clinical symptoms were rich and diverse, the duration of disease was long, and the prognosis was poor. Thus, the relevant ophthalmological examination and intervention treatment should be perfected as soon as possible. PMID: 33029389 [PubMed]
Source: Journal of Ophthalmology - Category: Opthalmology Tags: J Ophthalmol Source Type: research
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