Anti-inflammatory activities of isopimara-8(9),15-diene diterpenoids and mode of action of kaempulchraols B –D from Kaempferia pulchra rhizomes

AbstractKaempulchraols B –D (2 –4), isopimara-8(9),15-diene diterpenoids isolated fromKaempferia pulchra rhizomes collected in Myanmar, were identified as potent NF- κB inhibitors. These compounds were also effective as NO inhibitory agents, with IC50 values of 47.69, 44.97, and 38.17  μM, respectively, without showing any cytotoxicity against LPS-induced RAW264.7 cells. Investigations of the mechanisms of action of2 –4 revealed that they inhibit the NF- κB-mediated transactivation of a luciferase reporter gene, IL-6 production, and COX-2 expression, with an effective dose of 25 μM. Thus, isopimarane diterpenoids are suggested to be potent inhibitors of NF-κB pathways and could be further explored as potential anti-inflammatory lead compounds.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research