Genome editing of rodents by electroporation of CRISPR/Cas9 into frozen-warmed pronuclear-stage embryos.

In this study, we propose an ethanol-free, slow-freezing method to reduce physical damage to pronuclear-stage embryos followed by the TAKE method. All mouse and rat frozen embryos were survived after electroporation, and 18% and 100% of offspring were edited target gene, respectively. The resulting protocol is an efficient method for producing genome edited animals.
PMID: 31987837 [PubMed - as supplied by publisher]

Source: Cryobiology - January 23, 2020 Category: Biology Authors: Kaneko T, Nakagawa Y Tags: Cryobiology Source Type: research