Brucella melitensis omp31 mutant is attenuated and confers protection against virulent Brucella melitensis challenge in BALB/c mice.

Brucella melitensis omp31 mutant is attenuated and confers protection against virulent Brucella melitensis challenge in BALB/c mice. J Microbiol Biotechnol. 2020 Jan 17;: Authors: Verdiguel-Fernández L, Oropeza-Navarro R, Ortiz-Rico A, Robles-Pesina G, Ramírez-Lezama J, Castañeda-Ramírez A, Verdugo-Rodríguez A Abstract For control of brucellosis in small ruminants, attenuated B. melitensis Rev1 is used but it can be virulent for animals and human. Based on these aspects, it is essential to identify potential immunogens to avoid these problems on prevention of brucellosis. The majority OMP of the Omp25/31 family have been studied because these proteins contribute in a relevant way to the maintenance of the integrity of the outer membrane but its implication in the virulence of the different species of this genus is not clearly described. Therefore the aim of this work was to study the role of Omp31 on virulence by determining the residual virulence and detecting lesions in spleen and testis of mice inoculated with the B. melitensis LVM31 mutant strain in addition to evaluate the conferred protection in mice immunized with the mutant strain against the challenge with the B. melitensis Bm133 virulent strain. Our results showed that the mutation of omp31 caused a decrease in splenic colonization without generating apparent lesions or histopathological changes apparent in both organs in comparison with the control strains an...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research

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AbstractBrucellosis is a neglected zoonosis. It causes acute febrile illness and a potentially debilitating chronic infection in humans, and livestock infection has substantial socioeconomic impact. Over the past two decades, improvements have been made to better understand the various aspects of human and animal brucellosis. Meanwhile, especially in the developing world, immense challenges that remain in controlling and eradicating brucellosis are novel diagnostics tools and efficacious vaccines. Here, we will focus on the remarkable issues on epidemiological survey, as well as the priority and challenge of brucellosis in...
Source: Infectious Diseases of Poverty - Category: Infectious Diseases Source Type: research
by Carine Rodrigues Pereira, Jo ão Vitor Fernandes Cotrim de Almeida, Izabela Regina Cardoso de Oliveira, Luciana Faria de Oliveira, Luciano José Pereira, Márcio Gilberto Zangerônimo, Andrey Pereira Lage, Elaine Maria Seles Dorneles Brucellosis is a neglected zoonotic disease of remarkable importance worldwide. The focus of this systematic review was to investigate occupational brucellosis and to identify the main infection risks for each group exposed to the pathogen. Seven databases were used to identify papers related to o ccupational brucellosis: CABI, Cochrane, Pubmed, Scielo, Science Direc...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Brucella spp. are facultative intracellular bacteria notorious for their ability to induce a chronic, and often lifelong, infection known as brucellosis. To date, no licensed vaccine exists for prevention of human disease, and mechanisms underlying chronic illness and immune evasion remain elusive. We and others have observed that B cell-deficient mice challenged with Brucella display reduced bacterial burden following infection, but the underlying mechanism has not been clearly defined. Here, we show that at 1 month postinfection, B cell deficiency alone enhanced resistance to splenic infection ~100-fold; however, combine...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Microbial Immunity and Vaccines Source Type: research
Conclusions: The study concluded in bringing out the contributing risk factors for brucellosis. The study concluded that treating animal infection on their own and helping animals during reproduction without using protective gear, as major contributing risk for brucellosis. Other factors includes, keeping animals in close proximity during sleep, irregular vaccination, etc.
Source: Indian Journal of Community Medicine - Category: International Medicine & Public Health Authors: Source Type: research
In this study, the gene encoding OMP19 antigen was primarily amplified and cloned into an expression vector called pT1NX, and then transformed to L. casei cell via electroporation technique. The expression was confirmed using specific antibody against the recombinant protein via immunological screening tests such as western blot and immunofluorescence assay. Finally, recombinant L. casei was orally fed to mice and the results were further recorded, indicating that the mice group which received OMP19 through L. casei based vaccine represented a very good general and mucosal immune responses protective against challenges wit...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
ary U, Wensman JJ Abstract An extended range of host susceptibility including camel has been evidenced for some of the important veterinary and public health pathogens, such as brucellosis, peste des petits ruminants (PPR) and bluetongue (BT). However, in disease endemic settings across many parts of the globe, most of the disease control interventions accounts for small and large ruminants, whereas unusual hosts and/or natural reservoirs, such as camels, remain neglected for disease control measures including routine vaccination. Such a policy drawback not only plays an important role in disease epizootiology par...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
Publication date: Available online 20 February 2020Source: Research in Veterinary ScienceAuthor(s): Vinod Ahuja, David Ward, Rukhshona Rajabov, Anni McLeod, Ron JacksonAbstractIn the context of significant public health benefits of brucellosis control and shrinking public resources for livestock vaccination, this paper considers the willingness of small ruminant livestock owners to pay for vaccination of their animals against brucellosis. The willingness to pay is estimated through a binary choice contingent valuation approach using data from a rural household survey specially designed for this purpose. The survey was cond...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 31 January 2020Source: Journal of Microbiological MethodsAuthor(s): Triveni Kalleshamurthy, Somy Skariah, Yashaswini Rathore, Kavana D. Ramanjinappa, Chaitra Nagaraj, Bibek Ranjan Shome, Habibur Rahman, Nagendra Nath Barman, Rajeswari ShomeAbstractBrucellosis is an important zoonosis that constitutes a serious public health hazard which is caused by a bacterium belonging to the genus Brucella. In the present study, two highly specific serological tests for brucellosis diagnosis, fluorescence polarization assay (FPA) and competitive ELISA (cELISA) were standardized in the laboratory, evalu...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
AbstractMore than 70% of zoonotic diseases are wildlife associated putting wildlife professionals at increased risk of occupational exposure. In 2008 and 2018, the Arizona Department of Health Services surveyed Arizona wildlife professionals from multiple agencies to assess the risk of disease exposure, rabies pre-exposure prophylaxis (PrEP) history, personal protective equipment (PPE) use, and zoonoses knowledge. In 2008, a 12-question survey was distributed at a state wildlife professional meeting using an anonymous email link. In 2018, a 20-question survey was distributed using an anonymous email link to wildlife agency...
Source: EcoHealth - Category: Environmental Health Source Type: research
Publication date: Available online 26 December 2019Source: Veterinary MicrobiologyAuthor(s): Bisvanath Patra, Manjit Panigrahi, Sheikh Firdous Ahmad, Satyabrata Dandapat, Pushpendra Kumar, Bharat BhushanAbstractIn the present study, we analyzed the immune response of calves to Brucella abortus strain 19 vaccine (S19) and its association with MHC class I (BoLA-A) alleles (exons 2-3 and 4-5). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for typing of MHC class I BoLA-A exon 2-3 with DdeI and TaqI restriction enzymes and exon 4-5 with HinfI in 45 crossbred calves. The PCR-RFLP analysi...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
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