Purification and characterization of anti-tubercular and anticancer protein from Staphylococcus hominis strain MANF2: In silico structural and functional insight of peptide

Publication date: Available online 27 January 2020Source: Saudi Journal of Biological SciencesAuthor(s): Ameer Khusro, Chirom Aarti, B. Mahizhaveni, Azger Dusthackeer, Paul Agastian, Galal Ali Esmail, Abdul-Kareem Mohammed Ghilan, Naif Abdullah Al-Dhabi, Mariadhas Valan ArasuAbstractThe present context was investigated to purify and characterize anti-tubercular as well as anticancer protein from fermented food associated Staphylococcus hominis strain MANF2. Initially, the anti-tubercular potency of strain MANF2 was assessed against Mycobacterium tuberculosis H37Rv using luciferase reporter phase assay which revealed pronounced relative light unit (RLU) reduction of 92.5±1.2%. The anticancer property of strain MANF2 was demonstrated against lung cancer (A549) and colon cancer (HT-29) cell lines using MTT assay which showed reduced viabilities. Anti-tubercular activities of the purified protein were observed to be increased significantly (P<0.05) ranging from 34.6±0.3 to 71.4±0.4% of RLU reduction. Likewise, the purified protein showed significantly (P<0.05) reduced viabilities of A549 and HT-29 cancer cells with IC50 values of 46.6 and 48.9 µg/mL, respectively. The nominal mass of the purified protein was found to be 7712.3 Da as obtained from MALDI-TOF MS/MS spectrum. The protein showed the sequence homology with 1–336 amino acids of Glyceraldehyde-3-phosphate dehydrogenase from Staphylococcus sp., thus, categorizing as a new class of Glyceraldehyde-3-phosphate ...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research