Cytotoxicity and genotoxicity of cadmium oxide nanoparticles evaluated using in vitro assays

In this study, 10 nm CdO core-PEG stabilized NPs were synthesized, characterized and used for evaluation of CdO NPs’ cytotoxicity and genotoxicity. Release of cadmium ions (Cd+2) from the CdO NPs in cell culture medium, cellular uptake of the NPs, and the endotoxin content of the particles were measured prior to the toxicity assays. Cytotoxicity was evaluated using the MTS assay, ATP content detection assay, and LDH assay. Genotoxicity was assessed using the Ames test, Comet assay, micronucleus assay, and mouse lymphoma assay. The cytotoxicity of cadmium chloride (CdCl2) was also evaluated along with that of the CdO NPs. The results showed that endotoxin levels within the CdO NPs were below the limit of detection. CdO NPs induced concentration-dependent cytotoxicity in TK6 and HepG2 cells with the MTS, ATP and LDH assays. Although the genotoxicity of CdO NPs was negative in the Ames test, positive results were obtained with the micronucleus, Comet, and mouse lymphoma assays. The negative response of CdO NPs with the Ames test may be the result of unsuitability of the assay for measuring NPs, while the positive responses from other genotoxicity assays suggest that CdO NPs can induce chromosomal damage, single or double strand breaks in DNA, and mutations. The toxicity of the CdO NPs results from the NPs themselves and not from the released Cd+2, because the ions released from the NPs were minimal. These results demonstrate that CdO NPs are cytotoxic and genotoxic ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research

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Publication date: Available online 22 February 2020Source: Reproductive BioMedicine OnlineAuthor(s): Andrea BUSNELLI, Amerigo VITAGLIANO, Laura MENSI, Stefano ACERBONI, Alessandro BULFONI, Francesca FILIPPI, Edgardo SOMIGLIANAAbstractSeveral epidemiological studies investigated the effects of cancer therapies on fertility. However, conflicting data have been reported.The present systematic review and meta-analysis aims to determine the chances of childbirth in women survived from different types of cancer.We systematically searched Pubmed, MEDLINE, Embase and Scopus, from database inception to July 17, 2019, for published ...
Source: Reproductive BioMedicine Online - Category: Reproduction Medicine Source Type: research
Abstract Seven members of signaling lymphocytic activation molecule (SLAM) family receptors (SFRs) are ubiquitously expressed on hematopoietic cells and they play critical roles in immune cell differentiation and activation. The engagement of these receptors transmits intracellular signaling mainly by recruiting SLAM-associated protein (SAP) and its related adaptors, EWS-FLI1-activated transcript-2 (EAT-2) and EAT-2-related transducer (ERT). The critical roles of SFRs and SAP-family adaptors are highlighted by the discovery that SAP is mutated in human X-linked lymphoproliferative (XLP1) disease in which the conta...
Source: Advances in Immunology - Category: Allergy & Immunology Authors: Tags: Adv Immunol Source Type: research
ConclusionPRS was associated with increased risk of FL, but improved overall survival.
Source: Cancer Epidemiology - Category: Cancer & Oncology Source Type: research
ConclusionsWe confirm the high prevalence of ID and anemia in cancer patients. ID status is correlated to tumor response providing a strong rationale for iron monitoring during cancer management.
Source: Supportive Care in Cancer - Category: Cancer & Oncology Source Type: research
We present a case report of an adult woman with AKL-positive ALCL, diagnosed by endobronchial ultrasound-guided transbronchial needle aspirate (EBUS-TBNA).A 59-year-old women with no history of breast implants, was admitted for a four-month low back pain. Initially, the patient was treated for a spondyloarthropathy, but due to persistence of the symptoms, a lumbosacral MRI was performed, showing changes in morphology and signal intensity in the vertebral body of L3, along with edema and a paravertebral collection that affected the left psoas muscle, suggesting granulomatous spondylodiscitis. Chest CT-scan showed mild left ...
Source: Respiratory Medicine Case Reports - Category: Respiratory Medicine Source Type: research
The objective of this review is to provide a comprehensive summary of ICANS and strategies for prompt identification and management of patients presenting to the intensive care unit with this syndrome.
Source: Critical Care Nursing Quarterly - Category: Nursing Tags: Original Articles Source Type: research
ConclusionWhile reduced impact of a cancer diagnosis on LOLE over time is encouraging, the growing number of cancer survivors in Australia is likely to pose complex challenges for cancer patients, their care givers, and health-care systems.
Source: Cancer Epidemiology - Category: Cancer & Oncology Source Type: research
AbstractMarginal zone lymphomas (MZLs) are rare and indolent subtypes of non-Hodgkin lymphomas, and their clinical behaviours are heterogeneous. The aim of this study was to evaluate the clinical and prognostic characteristics of MZL. In this multicentre retrospective study, we analyzed demographical, clinical and prognostic features of 64 MZL patients. The median age was 54.0 and 78.1% of the patients had extra-nodal disease at presentation. Most of the patients were treated with chemotherapy. The 5  years and 10 years overall survival (OS) rates were 74.5% and 62.1%, respectively. The analysis of factors associ...
Source: Indian Journal of Hematology and Blood Transfusion - Category: Hematology Source Type: research
Conditions:   Small Lymphocytic Lymphoma;   Lymphoplasmacytic Lymphoma;   Marginal Zone Lymphoma;   Mantle Cell Lymphoma;   Diffuse Large B Cell Lymphoma Intervention:   Drug: YY-20394 Sponsor:   Shanghai YingLi Pharmaceutical Co. Ltd. Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Extranodal NK/T-cell Lymphoma Interventions:   Drug: Sintilimab;   Drug: Decitabine Sponsor:   Beijing Tongren Hospital Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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