Use of a plasma test for verifying epidermal growth factor receptor gene (EGFR) mutations in fluid samples from non-small cell lung cancer patients

Publication date: Available online 27 January 2020Source: Respiratory Medicine Case ReportsAuthor(s): Wataru Ogura, Kouki Ohtsuka, Masachika Fujiwara, Ryota Tanaka, Kumiko Sekiguchi, Hiroaki Ohnishi, Takashi WatanabeAbstractBecause epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) are effective in the treatment of non-small cell lung cancer (NSCLC) patients with EGFR mutations, it is critical to obtain accurate EGFR mutation test results. For NSCLC patients, EGFR mutation testing is performed using the commercial Cobas EGFR Mutation test v2.0, which can be used to analyze both formalin-fixed, paraffin-embedded (FFPE) tissue (FFPE test, or FT) and plasma samples (plasma test, or PT). Since primary tumor tissues are often unavailable from relapsed patients, fluid samples are often used for EGFR mutation testing, but they are often tested using the FT. Here, we report three cases in which EGFR mutations were detected using the FT with FFPE primary tumor tissue samples, but were not detected using fluid samples (two pleural effusion and one cerebrospinal fluid sample). Because the FT may not be capable of detecting EGFR mutations in fluid samples, we used the PT, which is more sensitive, to verify the presence of EGFR mutations using the same fluid samples. As expected, the PT detected the same EGFR mutations in fluid samples as the FT did in FFPE primary tumor tissue samples.
Source: Respiratory Medicine Case Reports - Category: Respiratory Medicine Source Type: research

Related Links:

Condition:   Non-small Cell Lung Cancer Stage I Intervention:   Combination Product: nivolumab Sponsor:   Hospital Israelita Albert Einstein Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Authors: Chang CP, Hu MH, Hsiao YP, Wang YC Abstract Suppression of tumorigenicity 2 (ST2), also known as interleukin-1 receptor-like 1 (IL1RL1), is one of the natural receptors of IL-33. Three major isoforms, ST2L (transmembrane form), sST2 (soluble form), and ST2V, are generated by alternative splicing. Damage to stromal cells induces necrosis and release of IL-33, which binds to heterodimeric ST2L/IL-1RAcP complex on the membrane of a variety of immune cells. This IL-33/ST2L signal induces transcription of the downstream inflammatory and anti-inflammatory genes by activating diverse intracellular kinases and fac...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
ConclusionsBoth pretreatment CT and CBCT radiomics features could predict disease progression and lung injury. A model with CBCT plus pretreatment CT radiomics features might improve the prediction of lung toxicity in comparison with a model with pretreatment CT features alone.Key pointsSignificant findings of the study: A model with cone ‐beam CT radiomics features plus pre‐treatment CT radiomics features might improve the prediction of lung toxicity after SBRT in stage I NSCLC patients.What this study adds: In the prediction of PFS and lung toxicity in early ‐stage NSCLC patients treated with SBRT, CBCT radiomics c...
Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: Original Article Source Type: research
ConclusionsPDW might act as an independent risk factor to predict progression and prognosis. Preoperative PDW combined with the TNM staging system showed a better ability to assess the prognosis of NSCLC patients.Key points Our study focused on the prognostic value of preoperative PDW in 750 patients with NSCLC. We also analyzed preoperative PDW in different stages and histological subtypes systematically. A model built by preoperative PDW combined with the TNM staging system had a better prognostic ability. LRT was used to calculate values of the goodness of fit between the model and the TNM staging system.
Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: ORIGINAL ARTICLE Source Type: research
BackgroundCD73 induces the dephosphorylation of adenosine monophosphate converting it to adenosine, enabling malignancies to escape from immune surveillance. Although CD73 overexpression has been reported to be a poor prognostic factor in several malignancies including non ‐small cell lung cancer (NSCLC), its predictive relevance in NSCLC patients receiving immune checkpoint inhibitors is unknown. The present research was conducted to investigate the prognostic significance of CD73 expression in NSCLC patients receiving immune checkpoint inhibitors (ICIs).MethodsWe screened 91 patients with advanced or recurrent NSCLC wh...
Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: ORIGINAL ARTICLE Source Type: research
ConclusionThe results showed thatAURKA,KIAA0101,CDC20,MKI67,CHEK1,HJURP, andOIP5 may be critical genes in the development and prognosis of NSCLC.Key pointsOur results indicated thatAURKA,KIAA0101,CDC20,MKI67,CHEK1,HJURP, andOIP5 may be critical genes in the development and prognosis of NSCLC. Our methods showed a new way to explore the key genes in cancer development.
Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: ORIGINAL ARTICLE Source Type: research
This article reviews the efficacy of icotinib in different solid tumors with different epidermal growth factor receptor alterations.
Source: Anti-Cancer Drugs - Category: Cancer & Oncology Tags: Review Articles Source Type: research
Authors: Zhao D, Liu H, Liu H, Zhang X, Zhang M, Kolluri VK, Feng X, He Z, Wang M, Zhu T, Yan X, Zhou Y Abstract Circular RNAs (circRNAs) represent a class of endogenous non-coding RNAs. Recently, it has been reported that circRNAs might serve as novel potential biomarkers for the diagnosis of cancer, including non-small cell lung cancer (NSCLC). Mutations in the ATP binding cassette subfamily A member 3 (ABCA3) have been increasingly associated with lung disease; however, roles for circRNAs at the ABCA3 locus have not been identified. To characterize novel biomarkers in NSCLC, a bioinformatics platform was used to...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Conclusions: These results suggest that FGFR1 amplification occurs more frequently in SCC and in Asians. FGFR1 amplification may be a potential new therapeutic target for specific patients and lung cancer subtypes. PMID: 32051701 [PubMed]
Source: Archives of Medical Science - Category: General Medicine Tags: Arch Med Sci Source Type: research
Authors: Nakashima K, Saruwatari K, Sato R, Imamura K, Kajihara I, Fukusima S, Saito T, Ishizuka S, Tamanoi D, Jodai T, Hamada S, Tomita Y, Saeki S, Ichiyasu H, Oya N, Ihn H, Sakagami T Abstract Radiation recall dermatitis (RRD) is an inflammatory reaction that occurs at previously irradiated skin regions after drug administration. We herein report a patient with non-small-cell lung cancer treated previously with thoracic radiotherapy who developed severe RRD induced by atezolizumab (anti-programmed death 1 ligand 1 [PD-L1] antibody). Immunohistochemistry of the skin biopsy showed dermatitis with infiltration of CD...
Source: Internal Medicine - Category: Internal Medicine Tags: Intern Med Source Type: research
More News: Cancer | Cancer & Oncology | Genetics | Lung Cancer | Non-Small Cell Lung Cancer | Respiratory Medicine