Cyclophosphamide in Drosophila promotes genes and transposable elements differential expression and mitochondrial dysfunction.

Cyclophosphamide in Drosophila promotes genes and transposable elements differential expression and mitochondrial dysfunction. Comp Biochem Physiol C Toxicol Pharmacol. 2020 Jan 23;:108718 Authors: Stoffel TJR, Segatto AL, Silva MM, Prestes A, Barbosa NBV, Rocha JBT, Loreto ELS Abstract Cyclophosphamide (CPA) is an alkylating agent used for cancer chemotherapy, organ transplantation, and autoimmune disease treatment. Here, mRNA sequencing and high-resolution respirometry were performed to evaluate the alterations of Drosophila melanogaster gene expression fed with CPA under acute (0.1 mg/mL, for 24 h) and chronic (0.05 mg/mL, for 35 days) treatments. Differential expression analysis was performed using Cufflinks-Cuffdiff, DESeq2, and edgeR software. CPA affected genes are involved in several biological functions, including stress response and immune-related pathways, oxi-reduction and apoptotic processes, and cuticle and vitelline membrane formation. In particular, this is the first report of CPA-induced mitochondrial dysfunction caused by the downregulation of genes involved with mitochondria constituents. CPA treatment also changed the transcription pattern of transposable elements (TEs) from the gypsy and copia superfamilies. The results presented here provided evidence of CPA mitochondrial toxicity mechanisms and that CPA can modify TEs transcription in Drosophila flies. PMID: 31982542 [PubMed - as supplied by pub...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - Category: Biochemistry Authors: Tags: Comp Biochem Physiol C Toxicol Pharmacol Source Type: research