Exploring the structure of a ruthenium acetate cluster for biological purposes

Publication date: Available online 27 January 2020Source: Inorganic Chemistry CommunicationsAuthor(s): Camila F.N. da Silva, Loyanne Carla Barbosa Ramos, Thomas N. Rohrabaugh, Jessica Vandervord, Roberto Santana da Silva, Claudia Turro, Sofia NikolaouAbstractThis work evaluates the cytotoxicity of [Ru3(μ3-O)(μ-OAc)5{μ-η1(C),η2(N,N)-phen}(py)2](PF6) (1), a triruthenium acetate cluster combined with an ortho-metallated 1,10-phenanthroline (phen) ligand, along with its interactions with biomolecules. In vitro cytotoxicity tests against the B16F10 (murine melanoma) and L929 (fibroblast) cell lines showed that 1 decreased cancer cell viability by 50% at 25 μM, while it requires 50 μM of free phen ligand to achieve the same effect. Importantly, 1 is not active against non-tumor cell model L929 up to 100 μM. Spectrophotometric titrations suggest that 1 and DNA interacted weakly through electrostatic attraction and semi-intercalation. Despite the presence of a planar and aromatic ligand, relative viscosity measurements are not consistent with the DNA intercalation of 1, presumably due to the small size of phenanthroline. The protein-binding ability of compound 1 was evaluated using human serum albumin (HSA) as a model and monitored by spectroscopic techniques, including steady-state emission and absorption, time-resolved luminescence, and circular dichroism, CD. Compound 1 quenches the HSA emission efficiently (Kap= 4.72 x 108 M-1, 298 K) mostly by static quenching, consisten...
Source: Inorganic Chemistry Communications - Category: Chemistry Source Type: research