A comparative study on EpCAM antibody immobilization on gold surfaces and microfluidic channels for the detection of circulating tumor cells

Publication date: April 2020Source: Colloids and Surfaces B: Biointerfaces, Volume 188Author(s): Didem Cetin, Meltem Okan, Erhan Bat, Haluk KulahAbstractDetection of circulating tumor cells (CTCs) from the bloodstream holds great importance to diagnose cancer at early stages. However, CTCs being extremely rare in blood makes them difficult to reach. In this paper, we introduced different surface modification techniques for the enrichment and detection of MCF-7 in microfluidic biosensor applications using gold surface and EpCAM antibody. Mainly, two different mechanisms were employed to immobilize the antibodies; covalent bonding and bioaffinity interaction. Self-assembled monolayers (SAMs) formed on the gold surfaces were treated further for the immobilization of the antibody. The bioaffinity-based studies were performed with streptavidin and biotinylated EpCAM over the SAM coated surfaces. The cell attachment events were monitored using fluorescent microscope. Comparisons were made considering the length and functional end of alkanethiols and the positioning of the antibody. Then, these methods were integrated into a microfluidic channel system. Surface characterizations were performed with X-ray Photoelectron Spectroscopy, Atomic Force Microscopy, and contact angle measurements. The selectivity studies were carried out with EpCAM negative K562 leukaemia cell lines and the experiments were repeated for different types of surfaces, such as glass and polymer. Studies showed th...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research

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Abstract TCM-based medications have been used for millennia in China and have always been "different" from current Western-based medicines in that they frequently are still mixtures of predominately plant products. From the early 20th Century, there has been a move to identify both the actual compounds in these mixes, and then over the past approximately 50years, to utilize early information for current diseases, with an example being artemisinin for treatment of malaria. Since that discovery, Western scientists, together with their Chinese counterparts, have begun to investigate how TCM compositions can...
Source: Advances in Pharmacology - Category: Drugs & Pharmacology Authors: Tags: Adv Pharmacol Source Type: research
Kalaivani S Subramanian, Sree Rekha Jinkala, Rakhee Kar, Debdatta Basu, Biswajit DubashiIndian Journal of Cancer 2020 57(1):105-108
Source: Indian Journal of Cancer - Category: Cancer & Oncology Authors: Source Type: research
Musiol Isocitrate dehydrogenases constitute a class of enzymes that are crucial for cellular metabolism. The overexpression or mutation of isocitrate dehydrogenases are often found in leukemias, glioblastomas, lung cancers, and ductal pancreatic cancer among others. Mutation R132H, which changes the functionality of an enzyme to produce mutagenic 2-hydroxyglutarate instead of a normal product, is particularly important in this field. A series of inhibitors were described for these enzymes of which ivosidenib was the first to be approved for treating leukemia and bile duct cancers in 2018. Here, we investigated the po...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
Conditions:   Chronic Lymphocytic Leukemia;   Chronic Myelogenous Leukemia, BCR-ABL1 Positive;   Plasma Cell Myeloma Interventions:   Behavioral: The DISCO App;   Behavioral: Usual Care Sponsor:   Barbara Ann Karmanos Cancer Institute Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conditions:   Chronic Lymphocytic Leukemia;   Small Lymphocytic Lymphoma;   Follicular Lymphoma;   Marginal Zone Lymphoma;   Mantle Cell Lymphoma;   Diffuse Large B Cell Lymphoma;   Advanced Solid Tumor;   Non-small Cell Lung Cancer;   Metastatic Melanoma Interventions:   Drug: BGB-10188;   Drug: Zanubrutinib;   Drug: Tislelizumab Sponsor:   BeiGene Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conditions:   Chronic Lymphocytic Leukemia;   Chronic Myelogenous Leukemia, BCR-ABL1 Positive;   Plasma Cell Myeloma Interventions:   Behavioral: The DISCO App;   Behavioral: Usual Care Sponsor:   Barbara Ann Karmanos Cancer Institute Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conditions:   Chronic Lymphocytic Leukemia;   Small Lymphocytic Lymphoma;   Follicular Lymphoma;   Marginal Zone Lymphoma;   Mantle Cell Lymphoma;   Diffuse Large B Cell Lymphoma;   Advanced Solid Tumor;   Non-small Cell Lung Cancer;   Metastatic Melanoma Interventions:   Drug: BGB-10188;   Drug: Zanubrutinib;   Drug: Tislelizumab Sponsor:   BeiGene Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
(University of Toronto) New live-cell drug discovery tool developed at the University of Toronto identifies two leukemia drugs and other small molecules as potential treatments for lung tumours that stopped responding to therapy.
Source: EurekAlert! - Cancer - Category: Cancer & Oncology Source Type: news
o Gangemi B-cell chronic lymphocytic leukemia (B-CLL) is the main cause of mortality among hematologic diseases in Western nations. B-CLL is correlated with an intense alteration of the immune system. The altered functions of innate immune elements and adaptive immune factors are interconnected in B-CLL and are decisive for its onset, evolution, and therapeutic response. Modifications in the cytokine balance could support the growth of the leukemic clone via a modulation of cellular proliferation and apoptosis, as some cytokines have been reported to be able to affect the life of B-CLL cells in vivo. In this review,...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
Authors: Siedlecka-Kroplewska K, Wozniak M, Kmiec Z Abstract Resveratrol is a naturally occurring polyphenolic compound present in many plant species and wine. It possesses a wide range of beneficial biological properties including anticancer activity. Resveratrol has been demonstrated to induce both autophagy and apoptosis in several human cancer cell lines. The aim of this study was to investigate whether resveratrol modulates autophagy and apoptosis in MOLT-4 human lymphoblastic leukemia and HL-60 human promyelocytic leukemia cells. Cell viability was evaluated by the neutral red uptake assay. Cell cycle distrib...
Source: Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research
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