Shotgun Proteomic Analysis of ESBL-Producing and Non-ESBL-Producing Klebsiella Pneumoniae Clinical Isolates
Publication date: Available online 25 January 2020Source: Microbiological ResearchAuthor(s): Shymaa Enany, Samira Zakeer, Ahmed A. SayedAbstractKlebsiella pneumoniae is a pathogenic bacterium that is responsible for a wide range of infections in humans. An increased rate of infections caused by multi-drug-resistant K. pneumoniae has been noted in the last two decades. The association between antimicrobial resistance and virulence is an important topic of study. Genomic tools have been used widely for the detection of virulence. In our study, we used proteomic analysis with mass spectrometry and bioinformatics tools to explore the virulence factors of both ESBL-producing and non-ESBL-producing K. pneumoniae and to determine the association between virulence and antimicrobial resistance in these clinical isolates. We have revealed different proteomic profiles and different pathways between the ESBL- and non-ESBL-producing groups. Many proteins involved in stress responses have been reported in the shared proteome between ESBL-and non-ESBL producers, such as ElaB protein, Lon protease, and universal stress proteins G and A. The virulence and pathogenicity of ESBL-producing bacteria were stronger than those of the non-ESBL-producing bacteria. Several unique virulence determinants were identified in ESBL-producing K. pneumoniae, such as proteins with lyase, catalase, isochorismatase, and oxidoreductase activity.
ConclusionThis study reports the first genome sequence of a multidrug-resistant M. hominis isolate in China. These data may help to understand the genomic features and antimicrobial resistance mechanisms of this pathogen.
ConclusionsFollowing penicillin V, we observed a remarkable enrichment of the aecal resistome, indicating that even narrow-spectrum antibiotics may have important consequences in selecting for a more resistant microbiome.
Publication date: Available online 14 February 2020Source: Journal of Global Antimicrobial ResistanceAuthor(s): Sinan Mermer, Tuncer Turhan, Elif Bolat, Sohret Aydemir, Tansu Yamazhan, Husnu Pullukcu, Bilgin Arda, Hilal Sipahi, Sercan Ulusoy, Oguz Resat SipahiAbstractObjectivesHerein, we aimed to compare the antibacterial activity of ceftaroline and vancomycin in the treatment of MRSA meningitis in experimental rabbit meningitis model.MethodsWe compared the antibacterial activity of ceftaroline with vancomycin in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) meningitis (induced by MRSA strain ATCC 433...
ConclusionsPublic AMR resilience, responsibility, and behavior compliance are influenced by cognitive constructs, which are liable to the appropriation of misconceptions, fallacies, and social behavior models obtained via information brokering. A cohesive multidisciplinary participatory approach to AMR management and interventional design that applies the influence of cognitive constructs to inform public AMR behavior compliance is recommended.
Authors: Gil-Gil T, Martínez JL, Blanco P Abstract Introduction: Stenotrophomonas maltophilia is a prototype of bacteria intrinsically resistant to antibiotics. The reduced susceptibility of this microorganism to antimicrobials mainly relies on the presence in its chromosome of genes encoding efflux pumps and antibiotic inactivating enzymes. Consequently, the therapeutic options for treating S. maltophilia infections are limited.Areas covered: Known mechanisms of intrinsic, acquired and phenotypic resistance to antibiotics of S. maltophilia and the consequences of such resistance for treating S. maltophilia ...
ConclusionIn this study, we illustrate the whole genome sequence of high biofilm-forming capacity, hypermucoviscous K. pneumoniae isolate KSH203 with capsular serotype K25 belonging to ST11 isolated from a patient in China, which carried a large number of antimicrobial resistance genes and virulence-associated genes. Future studies are needed to be aware of dissemination of this type of strain among environmental, animal and human isolates.
ConclusionTaken together, these results demonstrate that PAS resistance and mutations in thekatG gene, inhA promoter or oxyR–ahpC intergenic region in INH-resistant M. tuberculosis have little effect on pasiniazid susceptibility.
ConclusionsThis Delphi survey provided useful indicators for the implementation of antimicrobial stewardship principles in the clinical management of ABSSSI and offers interesting elements of discussion about the barriers existing in Europe for the optimal implementation of antimicrobial stewardship programs.
CONCLUSIONSThefosA3 gene is the primary mechanism of fosfomycin resistance in CRKP and can be transmitted widely by plasmid in hospitals. Mutations in murA and glpT were found in fosfomycin-resistant fosA3-negative CRKP.
Publication date: Available online 13 February 2020Source: Journal of Global Antimicrobial ResistanceAuthor(s): Pengtao Bao, Zengzhen Zhang, Weiguo Zhao, Ying Jiang, Di Wang