Two New Unspecific Peroxygenases from Heterologous Expression of Fungal Genes in Escherichia coli.

Two New Unspecific Peroxygenases from Heterologous Expression of Fungal Genes in Escherichia coli. Appl Environ Microbiol. 2020 Jan 24;: Authors: Linde D, Olmedo A, González-Benjumea A, Estévez M, Renau-Mínguez C, Carro J, Fernández-Fueyo E, Gutiérrez A, Martínez AT Abstract Unspecific peroxygenases (UPOs) constitute a new family of fungal heme-thiolate enzymes with high biotechnological interest. Although several thousand genes encoding hypothetical UPO-type proteins have been identified in sequenced fungal genomes and other databases, only a handful of UPO enzymes have been experimentally characterized to date. Therefore, gene screening and heterologous expression from genetic databases is a priority in the search for ad-hoc UPOs for oxyfunctionalization reactions of interest. Very recently, Escherichia coli production (as a soluble and active enzyme) of a previously described basidiomycete UPO has been reported. Here, we explored this convenient heterologous expression system to obtain the protein products from several putative UPO genes available. In this way, two UPOs from the ascomycetes Collariella virescens (syn. Chaetomium virescens) and Daldinia caldariorum were successfully obtained, purified, and characterized. Comparison of their kinetic constants for oxidation of model substrates revealed 7-20 fold higher catalytic efficiency of the latter enzyme oxidizing simple aromatic compounds (such as veratryl alcohol, napht...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research