An Industrially Viable Technique for Fabrication of Docetaxel NLCs for Oncotherapy

Publication date: Available online 24 January 2020Source: International Journal of PharmaceuticsAuthor(s): Kharkar Prachi Balaram, Talkar Swapnil Sunil, Patravale Vandana BharatAbstractThe aim of this study is to formulate thermodynamically stable docetaxel loaded nanostructured lipid carriers (DTX-NLCs) for parenteral cancer therapy with improved long term stability and better pharmacokinetic performance. DTX-NLCswere formulated using microemulsion(ME) template technique and characterized for particle size,entrapment efficiency (EE%), drug content and in vitro release kinetics. Further evaluation of NLCs was carried out by IR, DSC, and SEM analysis. The optimized formulation was subjected to stability studies as per ICH guidelines.In vivo pharmacokinetic studies were carried out using SD rats in comparison with marketed formulation (MF). The particle size of optimized formulation was found to be in the range of 150-180 nm with EE of 68-70%.SEM images illustrated spherical morphology.Invitro release of the formulation showed a sustained release of the drug from formulation compared to MF.Formulation was found to be stable for a period of 6 months at conditions of 30°C/ 65%RH, 40°C/ 75%RH, and 2-8°C.In vitro cytotoxicity studies exhibited better cytotoxic activity of DTX-NLCs than MF against various types of cancer cell lines.In vivo pharmacokinetic studies showed better performance with respect to half life, volume of distribution, etc. (*p<0.05, by unpaired t test) of...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research