Multigene typing and phylogenetic analysis of Fasciola from endemic foci in Iran.

Multigene typing and phylogenetic analysis of Fasciola from endemic foci in Iran. Infect Genet Evol. 2020 Jan 21;:104202 Authors: Javanmard E, Ohari Y, Sadeghi A, Cheraghipour K, Aghdaei HA, Mirjalali H, Zali MR, Itagaki T Abstract Fasciolosis is a public health problem originally transmitted from livestock. Although molecular analysis of nuclear and mitochondrial genes allow discrimination between the two known species of Fasciola, F. hepatica and F. gigantica, these markers do not permit the detection of hybrid forms. On the other hand, molecular analysis of the pepck and pold genes from Fasciola do permit the detection of hybrid isolates and this study has not yet been performed on specimens from human and domesticated animals in Iran. Therefore, the aim of this study was to molecularly analyze Fasciola isolates using both conventional and the two new genetic markers mentioned above. Fifty-three adult Fasciola worms were isolated from the livers of 28 domesticated animals, including sheep, cattle and goat. Moreover, an adult worm was obtained from the bile duct of an infected woman during endoscopic retrograde cholangio-pancratography (ERCP). Targeted fragments from the ITS-1, NDI and COX1 genes were amplified using specific primers. PCR products were sequenced and genetically analyzed using MEGA v.7 and dnaSP software. Additionally, pepck and pold regions were amplified and analyzed using multiplex PCR and RLFP-PCR, respectively....
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research