PEGylation-based strategy to identify pathways involved in the activation of apoptotic BAX protein

ConclusionPEGylation-based approach is proven useful to shield individual binding grooves of BAX from apoptotic stimuli. Groove engagement in CG of BAX is required for a full cBid-induced BAX activation. This study has identified differences in the pathways involved during the initiation of BAX activation by full-length cBid protein versus synthetic BimBH3-based peptides.General significanceOur finding is potentially valuable for therapeutic application as the pore-forming activity of 164-PEG is independent from the cBid-mediated apoptotic pathways, but can be administrated by the synthetic short peptides.Graphical abstract

Source: Biochimica et Biophysica Acta (BBA) General Subjects - January 26, 2020 Category: Biochemistry Source Type: research