Increased tubulointerstitial recruitment of human CD141hi CLEC9A+ and CD1c+ myeloid dendritic cell subsets in renal fibrosis and chronic kidney disease.

In this study, we developed novel protocols for extracting renal DC subsets from diseased human kidneys and identified, enumerated and phenotyped them by multi-colour flow cytometry. We detected significantly greater numbers of total DC, CD141(hi) and CD1c(+) myeloid DC (mDC) subsets in diseased biopsies with interstitial fibrosis than diseased biopsies without fibrosis or healthy tissue. In contrast, plasmacytoid DC numbers were significantly higher in the fibrotic group compared to healthy tissue only. Numbers of all DC subsets correlated with loss of kidney function, recorded as estimated glomerular filtration rate. CD141(hi) DC expressed CLEC9A, whilst the majority of CD1c(+) DC lacked expression of CD1a and DC-SIGN, suggesting these mDC subsets may be circulating CD141(hi) and CD1c(+) blood DC infiltrating kidney tissue. Our analysis revealed CLEC9A(+) and CD1c(+) cells were restricted to the tubulointerstitium. Notably, DC expression of the costimulatory and maturation molecule CD86 was significantly increased in both diseased cohorts compared to healthy tissue. TGF-β levels in dissociated tissue supernatants were significantly elevated in diseased biopsies with fibrosis compared to non-fibrotic biopsies, with mDC identified as a major source of this pro-fibrotic cytokine. Collectively, our data indicate that activated mDC subsets, likely recruited into the tubulointerstitium, are positioned to play a role in the development of fibrosis and thus, progression to chr...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research

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CONCLUSION: Remote ischemic preconditioning did not modify irisin or troponin I expression, independent of the time of collection. PMID: 31799981 [PubMed - as supplied by publisher]
Source: Jornal Brasileiro de Nefrologia - Category: Urology & Nephrology Tags: J Bras Nefrol Source Type: research
CONCLUSION: In this small pilot study, cholecalciferol treatment appeared to increase serum phosphorous concentrations compared to ergocalciferol. These observations may warrant further large-scale studies that are appropriately powered to validate such findings. PMID: 31804923 [PubMed - in process]
Source: J Pharm Pharm Sci - Category: Drugs & Pharmacology Authors: Tags: J Pharm Pharm Sci Source Type: research
Authors: Jha VK, Rajkamal T, Padmaprakash KV, Sharma DP PMID: 31801341 [PubMed - in process]
Source: Journal of the Association of Physicians of India - Category: General Medicine Tags: J Assoc Physicians India Source Type: research
Authors: Gupta R, Sharma KK, Soni S, Gupta N, Khedar RS Abstract Background: Hypertension is highly prevalent in India but frequency of resistant hypertension has not been well studied. Methods: We performed a registry-based study at a single center in patients with primary diagnosis of hypertension (n=3073). Details of co-morbidities, medications and blood pressure (BP) control were obtained. Patients with coronary heart disease, cerebrovascular disease and chronic kidney disease were excluded. Resistant hypertension was defined as uncontrolled hypertension (BP ≥140/90) with use of 3 drugs of which one was ...
Source: Journal of the Association of Physicians of India - Category: General Medicine Tags: J Assoc Physicians India Source Type: research
In this study, a combination of chromatography and mass spectrometry analysis has been used to characterise and compare the glycosylation profiles of five ESA products; Eprex® (epoetin alfa), NeoRecormon® (epoetin beta), Binocrit® (epoetin alfa biosimilar), Silapo (epoetin alfa biosimilar) and Aranesp® (darbepoetin alfa). The methods utilised include mixed-mode anion-exchange/hydrophilic interaction chromatography (AEX/HILIC-MS) for N-glycan identification and quantitation, and HILIC-MS for O-glycan characterisation. The products exhibit notable differences in N- and O-glycosylation, including attributes su...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research
Authors: Crawford DC, Lin J, Cooke Bailey JN, Kinzy T, Sedor JR, O'Toole JF, Bush WS Abstract Return of results is not common in research settings as standards are not yet in place for what to return, how to return, and to whom. As a pioneer of large-scale of return of research results, the Precision Medicine Initiative Cohort now known of All of Us plans to return pharmacogenomic results and variants of clinical significance to its participants starting late 2019. To better understand the local landscape of possibilities regarding return of research results, we assessed the frequency of pathogenic variants and APO...
Source: Pacific Symposium on Biocomputing - Category: Bioinformatics Tags: Pac Symp Biocomput Source Type: research
In this study, we used data mining techniques to search for subtypes in an unbiased fashion. Using electronic signatures of the disease, we identified a cohort of 13,290 patients with NAFLD from a hospital database. We gathered clinical data from multiple sources and applied unsupervised clustering to identify five subtypes among this cohort. Descriptive statistics and survival analysis showed that the subtypes were clinically distinct and were associated with different rates of death, cirrhosis, hepatocellular carcinoma, chronic kidney disease, cardiovascular disease, and myocardial infarction. Novel disease subtypes iden...
Source: Pacific Symposium on Biocomputing - Category: Bioinformatics Tags: Pac Symp Biocomput Source Type: research
Publication date: Available online 5 December 2019Source: Journal of the Formosan Medical AssociationAuthor(s): Chen-Ti Wang, Che-Wei Chang, Yung-Chuan Lu, Hing-Chung Lam, Shu-Ju Ku, Kuo-Bin Tseng, Yu-Hsi KaoSodium-glucose cotransporter-2 (SGLT-2) inhibitors have become the best choice of second-line oral antidiabetic drugs for patients with heart or chronic kidney disease. However, it is not clear how safe this treatment is for elderly patients, especially Asians, who are known to be more insulin deficient than Caucasian individuals with a similar insulin resistance. Here, we report a case concerning an elderly patient wi...
Source: Journal of the Formosan Medical Association - Category: General Medicine Source Type: research
Emerging evidence has demonstrated that epigenetic regulation plays a vital role in gene expression under normal and pathological conditions. Alterations in the expression and activation of histone methyltransferases (HMTs) have been reported in preclinical models of multiple kidney diseases, including acute kidney injury, chronic kidney disease, diabetic nephropathy, polycystic kidney disease, and renal cell carcinoma. Pharmacological inhibition of these enzymes has shown promise in preclinical models of those renal diseases. In this review, we summarize recent knowledge regarding expression and activation of various HMTs...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
The current session of American Heart Association in Philadelphia (November 16-18, 2019) was marked by considerable buzz around the presentation of International Study of Comparative Health Effectiveness With Medical and Invasive Approaches (ISCHEMIA) trial and its sub studies including quality of life and chronic kidney disease patients.   The slides are available for review on ischemiatrial.org albeit the full text of the publication is awaited. The excitement around the trial is understandable; the data surrounding revcascularization in stable coronary artery disease is derived from historical studies, some of...
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: Editorial Source Type: research
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